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Mechanisms of recruitment of the CTD phosphatase Rtr1 to RNA polymerase II
Rtr1 RNA polymerase II transcription
2015/5/25
The C-terminal domain (CTD) of the RNA polymerase II (RNAPII) subunit Rpb1 must exist in a hypophosphorylated state prior to forming a competent transcription initiation complex. However, during trans...
The role of the CTD phosphatase Rrt1 and post-translational modifications in regulation of RNA polymerase II
CTD phosphatase Rtr1 MuDPIT AP-MS
2015/5/25
RNA polymerase II (RNAPII) is regulated by multiple modifications to the C-terminal domain (CTD) of the largest subunit, Rpb1. This study has focused on the relationship between hyperphosphorylation o...
RNA-dependent RNA polymerase of turnip crinkle virus and the effect of coat protein on the replication of subviral RNAs
Biological sciences
2015/1/28
In this dissertation, I report the functions of the TCV coat protein in TCV viral and subviral RNA replication using in vivo and in vitro systems. In chapter 2, I present my results on construction of...
The stability of T7 RNA polymerase elongation complexes and promoter function analysis by in vitro selection
Forward translocation model polymerase complex stability DNA structure
2014/12/11
The forward translocation model (paused or stalled polymerase moves forward in the absence of transcription, dissociation of the complex happens when hybrid is too short to stabilize the complex) is t...
Functional tests of structural models for abortive cycling in T7 RNA polymerase
Pure sciences Biological sciences Abortive cycling RNA polymerase Transcription
2014/12/10
Transcription initiates when the enzyme binds to the promoter region and melts open an initial transcribing bubble that extends from position -4 to +4. The initiation phase continues until the enzyme ...
Phosphorylation Status of RNA Polymerase II Carboxyl-terminal Domain in Porcine Oocytes and Early Embryos
RNA Polymerase II Porcine Oocyte Embryos α-Amanitin
2016/5/10
Fertilization of the oocyte commences embryogenesis during which maternally inherited mRNAs are degraded and the embryonic genome is activated. Transcription of embryonic mRNA is initiated by embryoni...
期刊信息
篇名
A modified procedure for fast purification of T7 RNA polymerase
语种
英文
撰写或编译
撰写
作者
Yong Li,En-duo Wang(通讯作者),Ying-lai Wang
第一作者单位
中科院上海生化所分子生物学国家重点实验室
刊物名称
Protein Expression and Purification,
...