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One of the most expensive steps in manufacturing protein drugs such as antibodies or insulin is the purification step: isolating the protein from the bioreactor used to produce it. This step can accou...
PURIFICATION OF SIMPL ANTIBODY AND IMMUNOFLUORESCENCE OF SIMPL SUB-CELLULAR LOCALIZATION IN RESPONSE TO TNFα- AND IL-1
SIMPL Transcriptional Co-Activator NF-kB
2015/5/25
SIMPL is a transcriptional co-activator that alters the activity of transcription factor, NF-κB. In response to pathogens, cytokines such as Interleukin-1 (IL-1) and Tumor Necrosis Factor (TNF) signal...
Purification of Escherichia coli-expressed HIS-tagged Maedi-Visna p25 core antigen by Ni2+-chelate affinity chromatography
recombinant antigen ELISA immunoblo
2015/1/20
In this study, recombinant histidine tagged p25 capsid protein of Maedi-Visna virus was developed. Part of the viral genome coding p25 protein was positioned downstream and in frame with a metal bindi...
Expression and Purification of Human Lysosomal β-galactosidase from Pichia Pastoris
human β-galactosidase lysosomal storage diseases Pichia pastoris protein expression protein purification biochemistry and molecular biology
2014/12/12
Lysosomal storage diseases are genetically inherited diseases caused by the dysfunction of lysosomal enzymes. In a normal cell, lysosomal enzymes cleave specific macromolecules as they are transported...
Human Niemann-Pick Type C2 Disease Protein Expression, Purification and Crystallization
Niemann-Pick type C2 NPC2 Structure Expression Crystallization Purification
2014/12/12
Niemann-Pick type C2 (NPC2) protein is a soluble protein that binds unesterified cholesterol. The protein helps transport unesterified cholesterol in tandem with the membrane protein Niemann-Pick type...
Expression, Purification, and Characteristic of Tibetan Sheep Breast Lysozyme Using Pichia pastoris Expression System
Pichia pastoris Purification Secretory Expression Tibetan Sheep
2016/5/16
A lysozyme gene from breast of Tibetan sheep was successfully expressed by secretion using a-factor signal sequence in the methylotrophic yeast, Pichia pastoris GS115. An expression yield and specific...
Production, Purification and Characterization of Inulinase from a Newly Isolated Streptomyces sp. CP01
Characterization Inulinase Production Purification Streptomyces
2013/3/8
Inulinase is an enzyme catalyzing the hydrolysis of inulin, a plant reserve polysaccharide, into fructoses and fructooligosaccharides which are widely used as food additives. Here we report inulinase ...
Thermal analysis of organoclays and application for water purification
Thermal analysis organoclays application water purification
2012/3/30
In recent years, organoclays have become widely used in many industrial applications, and particularly they have been applied as adsorbents for water purification (de Paiva et al., 2008; Zhou et al., ...
Analysis on the Environment Conservation Value and Purification Capacity of Wetland Plants
Conservation Value Purification Capacity Wetland Plants
2011/12/20
The environmental conservation values of wetland plants were estimated in successive terms, by using the calculation model of ecosystem service values. The removal experiments of CODcr and TN in waste...
Distillation and purification of symmetric entangled Gaussian states
distillation purification symmetric entangled Gaussian
2010/11/8
We propose an entanglement distillation and purification scheme for symmetric two-mode entangled Gaussian states that allows to asymptotically extract a pure entangled Gaussian state from any input en...
Deterministic polarization-entanglement purification using spatial entanglement
Deterministic polarization-entanglement purification spatial entanglement
2010/11/8
We present an efficient entanglement purification protocol with hyperentanglement in which additional spatial entanglement is utilized to purify the two-particle polarization-entangled state. The bit-...
We consider the problem of improving noisy quantum measurements by suitable preprocessing
strategies making many noisy detectors equivalent to a single ideal detector. For observables per-taining to ...
Quantum state engineering, purification, and number resolved photon detection with high finesse optical cavities
Quantum state high finesse optical
2010/3/10
We propose and analyze a multi-functional setup consisting of high finesse optical cavities, beam splitters, and phase shifters. The basic scheme projects arbitrary photonic two-mode input states onto...
Purification of L-Lysine in Simulated Moving Bed and Fixed-Bed Chromatography
ion exchange chromatography simulated moving bed purification L-lysine
2009/11/18
L-Lysine was produced by a microbial process utilizing a Corynebacterium glutamicum (ATCC 21799) strain. L-Lysine was purified from the cultivated medium by fixed-bed and simulated moving bed （SMB） c...
Research on Purification of Attapulgite Clay and Adsorption Characteristics for Metal Cations
Acid Treatment Alkaline Treatment Attapulgite Clay Cation-Adsorption Purification
2011/11/22
Attapulgite clay(ATP) from Xuyi county of China was purified by a wet method then treated with NaOH and 1.0 mol/L, 2.0 mol/L and 3.0 mol/L solutions of HCl. Transmission electron microscope(TEM) and X...