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Origins of the Sensitivity of Molecular Vibrations to Electric Fields:Carbonyl and Nitrosyl Stretches in Model Compounds and Proteins
phosphoenolpyruvate carboxylase thermostable limited proteolysis flexible loop main chain connectivity
2016/5/24
The vibrational Stark effect (VSE) is the effect of an electric field on the vibrational spectrum. Typically, the largest change induced by an electric field is the linear frequency shift whose sensit...
Chemical cross-linking/mass spectrometry targeting acidic residues in proteins and protein complexes.
Chemical cross-linking/mass spectrometry targeting acidic residues proteins protein complexes.
2015/12/11
The study of proteins and protein complexes using chemical cross-linking followed by the MS identification of the cross-linked peptides has found increasingly widespread use in recent years. Thus far,...
Coacervation of Oppositely Charged Macromolecules, Micelles and Proteins: Disproportionation and Hierarchical Structures
coacervation complexation phase transitions
2014/11/20
Analogous disproportionation processes lead to similarities in the structures present at incipient coacervation to those within the subsequent dense phase. Coacervation in polyelectrolyte/mixed micell...
Structural Analysis of Proteins by Covalent Labeling and Mass Spectrometric Detection
mass spectrometry protein 3D structure
2014/11/20
Covalent labeling and mass spectrometry are seeing increased use together as a way to obtain insight into the 3-dimensional structure of proteins and protein complexes. Several amino acid specific (e....
Temperature-Dependence of the Amide-I Frequency Map for Peptides and Proteins
Amide-I Frequency map Infrared spectrum Molecular dynamics simulation
2012/4/5
In our recent work [Phys. Chem. Chem. Phys. 11, 9149 (2009)], a molecular-mechanics force field-based amide-I vibration frequency map (MM-map) for peptides and proteins was constructed. In this work, ...
Polydispersity as a Parameter for Indicating the Thermal Stability of Proteins by Dynamic Light Scattering
Polydispersity Parameter Indicating Thermal Stability Proteins
2010/9/13
A physical parameter for predicting the thermal stability of proteins was provided by a new approach using dynamic light scattering (DLS). The relationship between the melting point measured by differ...
Traceable Amino Acid Analyses of Proteins and Peptides by Isotope-Dilution Mass Spectrometry Using Precolumn Derivatization Reagent
Traceable Amino Acid Analyses Proteins and Peptides Isotope-Dilution Mass Spectrometry
2010/9/17
We have developed an accurate and traceable quantitation method of proteins and peptides by isotope-dilution mass spectrometry with the precolumn derivatization for hydrolyzed amino acid. This method ...