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Red重组系统及在微生物基因敲除中的应用--The Red Recombination System and Its Application to Gene Knock-out in Microorganism
Red重组系统 基因敲除 抗药性基因 Red recombination system gene knock-out resistant gene
2007/12/5
在完成了对各种微生物基因组的测序以后，功能基因学的研究变得尤为重要。研究基因功能最直接的方法便是将待研究的基因失活。最初构建基因突变体是采用大肠杆菌的RecA系统，但是RecA重组系统操作复杂，重组效率低。最近建立了Red重组系统，该系统由3个蛋白组成：α蛋白(即λ核酸外切酶)，β蛋白，Gam蛋白。应用Red系统进行基因敲除，可以直接利用线性打靶DNA，两侧同源臂长度在35~60 bp即可发生同源...
Screening and Characterization of Lactate Dehydrogenase-producing Microorganism
Lactobacillus Lactate Dehydrogenase Direct-fed Microbials
2016/4/18
The objective of this work was to isolate a microorganism, able to produce high lactate dehydrogenase (LDH) activity, for use as a microbial feed additive. The LDH is an important enzyme for lactate c...
Treatment of Microencapsulated β- Galactosidase with Ozone : Effect on Enzyme and Microorganism
Ozone Treatment Microencapsulation Lactase Milk
2016/4/8
The present study was designed to examine the effect of ozone treatment in microencapsulated ß-galactosidase on inactivation of the enzyme and sterilization of microorganism. The efficiency was t...