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Cloning and Characterization of an Endoglucanase Gene from Actinomyces sp. Korean Native Goat 40
Korean Native Goat Actinomyces sp Endo-β-1 4-glucanase Cellulase
2016/5/20
A gene from Actinomyces sp. Korean native goat (KNG) 40 that encodes an endo-β-1,4-glucanase, EG1, was cloned and expressed in Escherichia coli (E. coli) DH5α. Recombinant plasmid DNA from a positive ...
Molecular Cloning, mRNA Expression,and Localization of the G-protein Subunit Galphaq in Sheep Testis and Epididymis
Epididymis G-protein Subunit Galphaq [GNAQ] Sheep Testis
2016/12/28
The reproductive function of G-protein subunit Galphaq (GNAQ), a member of the G protein alpha subunit family, has been extensively studied in humans and rats. However, no data is available on its sta...
Molecular Cloning and Characterization of a New C-type Lysozyme Gene from Yak Mammary Tissue
Yak Mammary Tissue Milk Lysozyme Recombinant Protein
2016/5/19
Milk lysozyme is the ubiquitous enzyme in milk of mammals. In this study, the cDNA sequence of a new chicken-type (c-type) milk lysozyme gene (YML), was cloned from yak mammary gland tissue. A 444 bp ...
Cloning and characterization of thioredoxin peroxidase (TPx) in {\it Onchocerca volvulus\/}
Infection silk worm parasites prevent infection animal models and gene
2014/12/11
About 400 million individuals worldwide are currently infected with filarial parasites. To find a cure or prevention for the infections, studies of the mechanisms of pathogenesis and the survival stra...
Cloning and Molecular Characterization of Porcine β-casein Gene (CNS2)
Lactogenic Hormones HC11 Cells TATA Box Milk Protein Porcine Casein Gene
2016/5/9
The production of therapeutic proteins from transgenic animals is one of the most important successes of animal biotechnology. Milk is presently the most mature system for production of therapeutic pr...
Interspecies Somatic Cell Nuclear Transfer Technique for Researching Dog Cloning and Embryonic Stem Cells
Canine Interspecies Porcine Somatic cell Nuclear Transfer
2016/5/5
Large quantities of high-quality recipient oocytes with uniform cytoplasm are needed for research in the promising field of somatic cell nuclear transfer (SCNT) and embryonic stem cell research. In ca...
Cloning and Distribution of Facilitative Glucose Transporter 2 (SLC2A2) in Pigs
GLUT2 Molecular Cloning Small Intestine Pig
2016/5/5
Glucose is the main energy source for mammalian cells and its absorption is co-mediated by two different families of glucose transporters, sodium/glucose co-transporters (SGLTs) and facilitative gluco...
Cloning and Expression of FSHb Gene and the Effect of FSHβ on the mRNA Levels of FSHR in the Local Chicken
Chicken FSHb Gene Expression FSH-receptor
2016/5/4
Follicle-stimulating hormone (FSH) is a pituitary glycoprotein hormone that is encoded by separate alpha- and beta-subunit genes. It plays a key role in stimulating and regulating ovarian follicular d...
Molecular Cloning, Tissue Distribution and Expression of Porcine y+L Amino Acid Transporter-1
Cloning Pig Amino Acid Transporter y+LAT1 SLC7A7
2016/5/4
In this study, we cloned, sequenced and characterized porcine y+L Amino Acid Transporter-1 (y+LAT1). By screening a translated EST database with the protein sequence of the human y+LAT1 and by using r...
Cloning of OLR1 Gene in Pig Adipose Tissue and Preliminary Study on Its Lipid-accumulating Effect
OLR1 Pig Lipid Metabolism Adipose Tissue
2016/5/3
In this study we cloned and characterized a novel lipid-accumulating gene, the oxidized low-density lipoprotein receptor 1 (OLR1), which is associated with lipogenesis. We analyzed the gene structure ...
Molecular Cloning, Segmental Distribution and Ontogenetic Regulation of Cationic Amino Acid Transporter 2 in Pigs
Amino Acid Transporter CAT-2 SLC7A2 Ontogenetic Regulation Molecular Cloning Pig
2016/4/29
The goal of this study was to elucidate the expression and segmental distribution of the glomerular cationic amino acid metabolism transporter-2 (CAT-2) and thus to improve our understanding of porcin...
Cloning of Chicken Microsomal Glutathione S-transferase 1 Gene (MGST1) and Identification of Its Different Splice Variants
Chicken MGST1 RACE Splice Variant
2016/4/29
Mammal microsomal glutathione transferase 1 (MGST1) can conjugate many toxic or carcinogenic substances and depress oxidative stress. In this study, Chicken MGST1 and its variants were cloned for the ...
Cloning and Sequence Analysis of Glyceraldehyde-3-Phosphate Dehydrogenase Gene in Yak
Yak Glyceraldehyde-3-Phosphate Dehydrogenase gene Cloning Housekeeping Gene
2016/4/28
In order to study the biological function of gapdh gene in yak, and prove whether the gapdh gene was a useful intra-reference gene that can be given an important role in molecular biology research of ...
Cloning, Expression, and Regulation of Bovine Cellular Retinoic Acid-binding Protein-II (CRABP-II) during Adipogenesis
Bovine Adipocyte Differentiation Lipid-binding Protein Retinoic Acid Retinoic Acid Receptor Retinoid X Receptor
2016/4/28
The mammalian cellular retinoic acid-binding proteins, CRABP-I and CRABP-II, bind retinoic acid which acts as an inducer of differentiation in several biological systems. To investigate a possible rol...
Molecular Cloning, Tissue Distribution and Segmental Ontogenetic Regulation of b0,+ Amino Acid Transporter in Lantang Pigs
Cationic Amino Acid Transporter b0, +AT SLC7A9 Ontogenetic Regulation
2016/4/28
Cationic amino acid transporter b0,+AT (HGMW-approved gene symbol SLC7A9, solute carrier family 7, member 9) plays a crucial role in amino acid nutrition. In the present study, we describe the cloning...