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小麦白粉病是小麦上的一种重要病害。研究小麦白粉菌致病相关基因及其在致病过程中的作用，对于丰富小麦白粉菌致病的分子机制和筛选防治新靶标具有重要意义。前期转录组测序结果提示一个小麦白粉菌ADP/ATP载体蛋白（ADP/ATP carrier protein，AACP）在小麦与白粉菌互作时上调表达。本研究利用cDNA末端快速克隆技术（rapid\|amplification of cDNA ends，R...
甘蔗ADP/ATP转运蛋白酶基因克隆及其序列的生物信息学分析
甘蔗 ADP/ATP转运蛋白酶 能量代谢 生物信息学
2009/11/5
【研究目的】甘蔗(Saccharum officinarum Linn) 是C4光合途径作物，有着特殊的能量代谢系统，研究其能量代谢过程的ADP/ATP转运蛋白酶基因具有重要的意义。【方法】本研究采用同源克隆原理和PCR技术克隆甘蔗AAC转运蛋白酶基因，并应用生物信息学方法，对其进行多序列比对分析、物理性质分析、三级结构预测、亚细胞定位、跨膜区域预测和疏水性分析。【结果】克隆获得甘蔗AAC cDN...
Regulation of Expression of D3-type Cyclins and ADP-Glucose Pyrophosphorylase Genes by Sugar,Cytokinin and ABA in Sweet Potato (Ipomoea batatas Lam.)
Abscisic acid ADP-glucose pyrophosphorylase cytokinin D3-type cyclin gene expression Ipomoea batatas sugar tuberous root formation
2010/3/4
The productivity of sweet potato is governed by both the rate of cell division and sink activity of its tuberous root. The aim of this study was to reveal the mechanisms that regulate cell division ac...
Relationship between Starch Content and Activity of Starch Synthase and ADP-glucose Pyrophosphorylase in Tuberous Root of Sweet Potato
ADP-glucose pyrophosphorylase Ipomoea batatas Starch content Starch synthase Sweet potato
2009/8/5
Relationship between starch content and the activity of starch synthase and ADP-glucose pyrophos-phorylase in tuberous roots of sweet potato was investigated using several cultivars. No correlation wa...
Isolation and Characterization of an ADP-Glucose Pyrophosphorylase Gene from Gossypium hirsutum L.
ADP-Glucose Pyrophosphorylase Gene Gossypium hirsutum L.
2009/4/30
Roots and stems of cotton (Gossypium hirsutum L.) plants store photoassimilate as starch. Partitioning of fixed carbon between starch in vegetative storage tissues and seed is likely to impact cotton ...
【目的】克隆甘蓝型油菜（Brassica napus L.）矮化突变体“NDF-1”和野生型近等基因系亲本“3529”中ADP-核糖基化因子（ADP-ribosylation factor，ARF）基因全长cDNA，分析矮化突变体中该基因在不同组织中的表达水平，探讨矮化突变体中该基因在甘蓝型油菜茎杆发育过程中的作用。【方法】采用SSH（suppression subtractive hybridi...