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UVR8 interacts with de novo DNA methyltransferase and suppresses DNA methylation in Arabidopsis
DNA methylation Light responses Plant signalling
2023/12/11
DNA methylation is an important epigenetic gene regulatory mechanism conserved in eukaryotes. Emerging evidence shows DNA methylation alterations in response to environmental cues. However, the mechan...
De novo phasing resolves haplotype sequences in complex plant genomes
de novo phasing haplotype-resolved assembly autopolyploid chromosomal rearrangement
2023/11/28
Genome phasing is a recently developed assembly method that separates heterozygous eukaryotic genomic regions and builds haplotype-resolved assemblies. Because differences between haplotypes are ignor...
南京农业大学农学院《Plant Cell》发表宋庆鑫教授团队“Asymmetric variation of DNA methylation during domestication and de-domestication of rice”（图）
宋庆鑫 水稻栽培 基因
2023/10/28
水稻是全球最为重要的粮食作物之一，在其漫长的驯化过程中形成了形态各异的水稻栽培亚型，包括粳稻、籼稻、Aro和Aus等。在水稻栽培种植历史过程中，有些水稻逐渐恢复部分野生稻表型特征，最终演化成严重威胁全球水稻产量的杂草稻，这种现象称之为“去驯化”。通过基因组进化研究发现，大量驯化位点的变异在去驯化过程中并不能得到恢复，这就暗示着可能存在别的遗传机制来调控杂草稻在去驯化过程中恢复野生稻表型。DNA甲基...
南京农业大学农学院《Nature Communications》上发表甘祥超教授团队“GALA: a computational framework for de novo chromosome-by-chromosomeassembly with long reads”（图）
甘祥超 植物育种 动物基因
2023/10/28
2023年2月22日，南京农业大学农学院甘祥超教授与其原德国马克斯·普朗克植物育种研究所团队在《Nature Communications》上发表题为“GALA: a computational framework for de novo chromosome-by-chromosomeassembly with long reads”的研究论文，并发布了相应的开源软件GALA (http://g...
桃树枝条皮层响应低温的特异蛋白质2-DE分析
桃 双向电泳(2-DE) 特异蛋白 抗寒性
2021/4/2
低温是限制桃树生长、发育、繁殖以及地理分布的一个重要环境因子。明确桃响应低温胁迫的分子调控机制有可能为培育和筛选出相对抗寒的桃品系提供研究基础。以抗寒性较强的秋燕桃为试材，将一年生休眠枝条置于-5（CK），-15，-25℃低温处理48 h，应用双向电泳技术（Two-dimensional electrophoresis，2-DE）测定枝条皮层蛋白质表达变化情况。结果显示：通过质谱鉴定发现，有150...
应我校生命科学学院的邀请，荷兰瓦赫宁根大学Wietse de Boer教授于2018年10月30日上午在生科院作了题为“Soil's Biocontrol Force”的学术报告。我院相关领域的教师和研究生一百余人参加了此次报告会。Wietse de Boer教授是荷兰皇家科学院生态研究所高级研究员，荷兰瓦赫宁根大学土壤生物系教授，长期从事土壤中细菌—真菌互作、土壤抑真菌机制等方面研究，在Natu...
荷兰皇家科学院生态研究所Wietse de Boer教授等4人访问中国科学院南京土壤研究所（图）
荷兰皇家科学院生态研究所 Wietse de Boer 教授 中国科学院南京土壤研究所
2018/11/5
2018年10月26日至31日，应中国科学院南京土壤研究所王兴祥研究员邀请，荷兰皇家科学院生态研究所Wietse de Boer教授、中国农科院植保所郭荣君副研究员、陕西省生物农业研究所白娅妮博士、西南大学植保学院刘晓娇博士等一行4人到访中国科学院土壤环境与污染修复重点实验室，并分别作了学术报告。所内60余名师生参加了报告会。报告中，Wietse de Boer教授介绍了荷兰皇家科学院生态研究所以...
人心果转录组De novo组装及奇可胶途径相关基因的分析
人心果 转录组 奇可胶
2018/8/1
为了探索人心果（Manilkara zapota L.）的转录组及奇可胶合成相关的基因，使用Illumina测序平台，对人心果果实、树皮和叶片分别进行转录组测序，使用Trinity等软件进行De novo组装和注释以及计算表达量，采用实时荧光定量PCR对转录组数据进行验证。经过组装得到162 455条unigene，总长度达139 792 553 nt，平均长度达861 nt，N50达1 544 ...
利用RNA seq技术对中华蜜蜂（Apis cerana cerana，简称中蜂）幼虫肠道参考转录组进行de novo组装，并进行功能及代谢通路注释，进而利用该转录组数据进行中蜂幼虫的SSR分子标记鉴定。【方法】实验室条件下饲养中蜂幼虫，将纯化的蜜蜂球囊菌（Ascosphaera apis，简称球囊菌）孢子饲喂3日龄幼虫，剖取4、5和6日龄幼虫肠道，液氮速冻。将健康幼虫肠道与感染球囊菌的幼虫肠道同...
Comparison of field efficacy of four natural fungicides and metiram against late blight (Phytophthora infestans [Mont.] de Bary) on tomato–Short Communication
abiotic factors biotic factors Lycopersicum esculentum Phytophthora infestans natural substances yield
2015/12/30
Fungicidal activity of asaronaldehyde, soybean lecithin, garlic extract, salicylic acid, and metiram against late blight on tomato was investigated in a field block experiment. Significantly the lowes...
Occurrence of Sclerotinia sclerotiorum (Lib) de Bary and Diaporthe (Phomopsis) helianthi Munt.-Cvet. et al. on Iva xanthiifolia Nutt. in Slovak Republic
Sclerotinia sclerotiorum Diaporthe (Phomopsis) helianthi
2015/3/6
The polyphagous pathogen Sclerotinia sclerotiorum (Lib) de Bary causes significant diseases of sunflower and
oil-seed rape while Diaporthe wilt, invoked by the pathogen Diaporthe (Phomopsis) heliant...
Sensitivity to fungicides in the isolates of Phytophthora infestans (Mont.) de Bary in the Czech Republic from 2003 to 2008
late blight fungicide resistance
2015/3/6
In the growing seasons from 2003 to 2008, 547 isolates of Phytophthora infestans from five regions in the Czech
Republic were collected and examined for their sensitivity to the active ingredients (...
Mating Type Distribution of Phytophthora infestans (Mont.) de Bary in the Czech Republic in 2007 and 2008
late blight pathogen pairing test
2015/3/5
A total of 195 isolates of Phytophthora infestans were collected from late blight-diseased potatoes grown in
several localities in the Czech Republic during the years 2007–2008. The isolates were an...
Approximation of Subsurface Drainage Discharge by De Zeeuw-Hellinga Theory and its Verification in Heavy Soils of Fluvial Landscape of the Cerhovice Brook
subsurface pipe drainage system subsurface drainage discharge
2015/3/1
The subsurface drainage discharge is one of the most important indicators of the impact of the drainage systems on the water management. The procedure adopted in this study is based on the application...
Occurrence and distribution of mating types A1 and A2 of Phytophthora infestans (Mont.) de Bary in the Czech Republic
potato late blight mating types PCR CAPs
2015/2/6
A total of 199 Phytophthora infestans isolates were obtained from leaves, tubers and fruits of infected crops of potato and tomato in different regions of the Czech Republic in 2003, 2004 and 2005. Th...