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The present experiment was designed to investigate a possibility of the in vitro cultured somatic cells to produce the germ line interspecific chimera in bird. The frozen-thawed fibroblast-like cells ...
Restriction of Germline Proliferation by Soft X-ray Irradiation of Chicken Embryos and its Application to Chimera Production
blastoderm chimera embryo culture primordial germ cells soft x-ray
2009/7/30
Primordial germ cells (PGCs) are the progenitor cells of gametes. Avian PGCs are located in the central region of the area pellucida at the blastoderm stage. PGCs enter the circulation soon after the ...
The Confirmation of Ploidy Periclinal Chimera and its Morphological Characteristics in Meiwa Kumquat‘Yubeni’
bud mutation chromosome observation flow cytometry histogenic layer polyploidy breeding
2009/7/7
The cultivar ‘Yubeni’ was discovered as a mutant of Meiwa kumquat (Fortunella crassifolia Swingle) in Kushima city, Miyazaki. According to the results of flow cytometry analysis and morphological inve...