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Molecular cloning and characterization of allele d‒a newly identified allele of the 1-aminocyclopropane-
Malus floribunda 821 polymorphism of ACO1 gene ripening ethylene
2016/6/3
Apple is a climacteric fruit of economic importance and high nutritional value. Better understanding of the apple ripening process is needed to improve fruit quality and extend apple shelf life. This ...
Cloning and Expression of Yak Active Chymosin in Pichia pastoris
Active Chymosin Yak Expression Pichia pastoris
2016/11/10
Rennet, a complex of enzymes found in the stomachs of ruminants, is an important component for cheese production. In our study, we described that yak chymosin gene recombinant Pichia pastoris strain c...
Cloning and sequencing of the complicated rDNA gene family of Bos taurus
GC-rich repetitive sequences PCR rDNA rRNA Bos Taurus
2015/6/2
The rDNA genes coding for ribosomal RNA (rRNA) in animals are repeat sequences with high GC content and complicated structure. Based on the sequences of human ribosomal DNA repeat unit and transcripti...
Isolation, cloning and sequence analysis of lactate dehydrogenase gene from Theileria annulata may lead to design of new antitheilerial drugs
Theileria annulata theileriosis antitheilerials lactate dehydrogenase gene cloning
2015/4/15
Theileriosis is a serious animal disease that is transmitted by ticks. Theileria species are tick-borne obligate intracellular protozoan parasites that cause severe and mild infections in their hosts....
Mass cloning of Rose and Mussaenda, popular garden plants, via somatic embryogenesis
in vitro ornamental plants somatic embryogenesis
2015/3/3
Protocols were developed for propagation of Rosa hybrida cv. Landora and Mussaenda erythrophylla cv. Rosea via somatic embryogenesis by manipulating growth regulators and culture conditions. Calli wer...
Molecular cloning of a phosphotriesterase-related protein gene of silkworm and its expression analysis in the silkworm infected with Bombyx mori cytoplasmic polyhedrosis virus
Silkworm Cytoplasmic Polyhedrosis Virus Phosphotriesterase-Related Protein Gene
2013/3/5
Bombyx mori cytoplasmic polyhedrosis virus is one of the major viral pathogens for the silkworm. The immune response of silkworm to the virus infection is obscure. A phosphotriesterase-related protein...
Cloning, Expression and Antifungal Activity of an Endochitinase Gene Derived from Barley (Hordeum Vulgare)
Barley endochitinase inclusion bodies Hordeum vulgare antifungal activity
2010/11/15
Fungal phytopathogens pose serious problems worldwide in the cultivation of economically
important plants, especially in the tropical and subtropical regions. Plants respond to fungal pathogens by pr...
Cloning, characterization, and expression of the gene encoding polygalacturonase- inhibiting proteins from strawberry
Strawberry polygalacturonase-inhibiting proteins (PGIP) cDNA cloning, tissue expression
2010/10/29
Polygalacturonase-inhibiting proteins (PGIP) play important roles in the defense against plant pathogens, especially fungi. The sequence of strawberry PGIP gene was obtained using reverse transcriptio...
Molecular Cloning and mRNA Expression of the Porcine Insulin-responsive Glucose Transporter (GLUT4)
GLUT4 IR Skeletal muscle Porcine
2016/5/4
Insulin-responsive glucose transporter 4 (GLUT4) is a member of the glucose transporter family and mainly presents in skeletal muscle and adipose tissue. To clarify the molecular structure of porcine ...
Cloning and characterization of the first actin gene in Chinese oak silkworm, Antheraea pernyi
Antheraea pernyi actin cloning expression pattern
2011/1/11
The first actin gene in Chinese oak silkworm, Antheraea pernyi (Apactin) was cloned and characterized. Remarkably, two types of the Apactin cDNA clones were isolated from the pupal cDNA library, which...
cDNA Cloning and mRNA Expression of Estrogen Receptor α in Japanese Quail
estrogen receptor α mRNA expression quail northern blot analysis RT-PCR
2009/7/31
The biological actions of estrogen are mediated via estrogen receptors (ER). Although chicken ER cDNA for subtypes α and β have been identified, only the β form of ER cDNA was cloned in quail. Hence, ...
cDNA Cloning and mRNA Expression of Androgen Receptor in Male Japanese Quail (Coturnix coturnix japonica)
androgen receptor castration day-lengths mRNA expression quail
2009/7/30
The biological activities of androgen are largely mediated via androgen receptor (AR). Although a partial sequence AR cDNA was revealed in canaries and zebra finch, AR cDNA has not been cloned in quai...
Cloning of Perivitelline Membrane Protein; ZP1 in Turkey (Meleagris gallopavo)
egg envelope fibrogenesis perivitelline membrane turkey ZP1
2009/7/29
In aves, the perivitelline membrane is an extracellular matrix surrounding the oocyte at the time of ovulation, and is composed of several glycoproteins belonging to the zona pellucida (ZP) gene famil...
Cloning and Expression of Cellular Glutathione Peroxidase (GPX1) in Japanese Quail (Coturnix japonica)
antioxidant enzyme cellular glutathione peroxidase Japanese quail mRNA expression selenocysteine
2009/7/29
Glutathione peroxidase 1 (cellular glutathione peroxidase, EC 1.11.1.9 (GPX1)) is an antioxidant enzyme that plays an important role in scavenging reactive oxygen species to protect cells from oxidati...
Cloning and Comparison of Prolactin Promoter in Galliformes
Galliformes prolactin promoter
2009/7/29
To investigate the mechanism regulating transcription of the prolactin (PRL) gene in avian species, the PRL promoter region in Ceylon junglefowl, Japanese quail, ring-necked pheasant, turkey, Indian p...