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搜索结果: 1-15 共查到农学 microsatellite markers相关记录21条 . 查询时间(0.07 秒)
Based on DNA analysis using 8 microsatellite markers, observed heterozygosity levels were similar to those expected in livestock populations subjected to selective breeding (0.530–0.664), and...
The genetic diversity was studied using microsatellite DNA markers in Laoshan dairy goat (LS), Xinong Saanen dairy goat (SN), Guanzhong dairy goat (GZ), Banjiao goat (BJ) and Guizhou white goat (GW). ...
Genetic diversity and genetic relationships of Czech apple cultivars were evaluated. Trees of 33 Czech apple cultivars and 97 reference foreign cultivars were analysed using the set of 10 SSR (simple ...
Genetic variability among 41 accessions of red pepper (Capsicum annuum L.) was assessed using eight microsatellite markers. Three of the microsatellite markers (Hpms 1-1, Hpms 1-168, and Hpms 1-274) h...
In order to evaluate the genetic diversity and discrimination among five Korean native chicken lines, a total of 86 individuals were genotyped using 150 microsatellite (MS) markers, and 15 highly poly...
Products made from wheat are the most important components of the human diet, and could also become a source of functional foods and feed ingredients, e.g. minerals, vitamins and/or phytochemicals. Th...
To estimate the genetic diversity of chickpea germplasm from Iran, a total of 307 landraces from 4 regions including: northern areas (29 from Ardebil, 3 from Qazvin and 5 from Mazanderan provinces), t...
We investigated paternal contribution in artificial crossed-populations made by mixed pollen from plus tree clones of Cryptomeria japonica D. Don in Niigata Prefecture. Using six polymorphic microsate...
The objective of this work was to develop polymorphic microsatellite markers derived from Japanese quail embryonic and cardiac cDNA libraries and to locate these markers on Kobe-NIBS Japanese quail (K...
Microsatellite markers are tandem repeats of short nucleotides which are abundant, evenly distributed and highly polymorphic in eukaryotic genomes. As such, they are a useful tool in poultry breeding....
Abdominal fat characters are complex and economically important in the poultry industry. Their selection may benefit from the implementation of marker-assisted selection (MAS). The objective of this s...
An enrichment library of GATA-repeats from genomic DNA was constructed in this study to isolate and characterize microsatellite loci in Tsaiya duck (Anas platyrhynchos). Thirty-three microsatellite ma...
摘要采用多重PCR的方法,以快速扩增微卫星标记和节约试剂为目标,对其反应条件进行优化后,获得了46个扩增效果理想的微卫星标记多重PCR组合,其中30个为二重PCR,16个为三重PCR。实验结果表明,这些多重PCP反应的引物浓度为0.06~0.3μmol/L, Mg2+的浓度变化范围为1.5~3.0mmol/L,采用的PCR缓冲液的倍数为1.0、1.2、1.4或1.6,每个PCR反应聚合酶的用量在0...
摘要采用13号染色体上与K88ab和K88ac受体基因连锁的2对引物(S0223和S0068),研究沙子岭猪和大约克猪的遗传差异性。结果表明,2个猪种在2个基因座均存在多态性,其基因杂合度和Shannon信息指数存在很大差异,而中外猪种的K88ab和K88ac受体基因也存在遗传差异,这2对引物可望作为K88ab和K88ac受体基因的遗传标记。Abstract: The genetic variat...
摘要 经多重PCR扩增20个微卫星标记,对凉山半细毛羊进行亲权鉴定。荧光标记检测扩增产物,并用Cervus 2.0分析。结果表明:当双亲未知时,累积排除概率为0.998666 ,在父系半同胞基础上进行母子亲权鉴定,累积排除概率为0.999994,置信度为95%,最终从8个候选父亲中找出215个后裔的父亲,又从147个候选母亲中找出它们的母亲,构建了一个混合家系系谱,为进行连锁分析、QTL定位...

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