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Identification of candidate genes for key fibre-related QTLs and derivation of favourable alleles in Gossypium hirsutum recombinant inbred lines with G. barbadense introgressions
海岛棉 基因组组分 遗传作图群体
2024/5/17
该研究以渗入了海岛棉基因组组分的优质种质鲁原343为亲本，构建了包含239个重组自交系的遗传作图群体，利用SLAF标签构建了高密度遗传图谱，结合7个环境的表型数据，共鉴定104个纤维品质和产量性状QTLs，其中66个QTL共定位于19个QTL簇中。该研究团队通过进一步对鲁原343进行全基因组深度测序，解析鲁原343的遗传组成及其对纤维相关性状的遗传贡献，发现来自G. hirsutum races（...
Genome-wide quantitative trait loci reveal the genetic basis of cotton fiber quality and yield-related traits in a G.hirsutum recombinant inbred line population
0-153 sGK9708 棉花产量 品质性状
2024/5/17
利用0-153和sGK9708为亲本构建的RIL群体为材料，通过全基因组QTL挖掘明确了棉花产量及品质性状的相关性及其与QTL簇加性效应方向的关系，初步揭示了棉花产量纤维品质性状负相关形成的遗传基础，为进一步利用标记辅助选择、基因组编辑等技术同步改良棉花纤维品质和产量提供了重要的基因资源和位点信息。该研究以0-153*SGK9708为亲本构建的含有196个重组自交系群体为材料，在黄河流域及西北内陆...
Quantitative trait loci for resistance to Sharp Eyespot (Rhizoctonia cerealis) in recombinant inbred wheat lines from the cross Niavt 14 × Xuzhou 25
genetic map molecular marker QTL Rhizoctonia cerealis Sharp Eyespot wheat
2016/12/8
This paper studies QTL (quantitative trait locus) resistance to Sharp Eyespot in wheat using a genetic population of the recombinant inbred line (RIL) hybridized from Niavt 14 and Xuzhou 25. Based on ...
Determination of exposure to Fasciola hepatica in horses from Uruguay using a recombinant-based ELISA
Fasciola hepatica equine Uruguay sensitisation FhrAPS
2015/10/9
The risk of exposure to Fasciola hepatica in horses from Uruguay was evaluated using ELISA and a recombinant surface protein (FhrAPS). Blood samples were collected from 368 horses from different distr...
Recombinant human activin A promotes development of bovine somatic cell nuclear transfer embryos matured in vitro
activin A SCNT embryo development bovine
2015/6/8
To improve the culture system of bovine somatic cell nuclear transfer (SCNT) embryos, we studied the effects of activin A on developmental competence of bovine SCNT embryos during the early developmen...
Production of scFv recombinant fragments against 2,4-dichlorophenoxyacetic acid hapten using na飗e phage library
na飗 e phage library selection phage antibody
2015/1/27
Three single chain variable fragment (scFv) antibodies against 2,4-dichlophenoxyacetic acid (2,4-D) herbicide were produced by the Griffin1.library. The selection of the scFv from the phage library wa...
Antibacterial Activity of Recombinant Pig Intestinal Parasite Cecropin P4 Peptide Secreted from Pichia pastoris
Cecropin Ascars suum Pichia pastoris Methanol Antimicrobial Activity
2016/5/16
Cecropins (Cec) are antibacterial peptides and their expression is induced in a pig intestinal parasite Ascaris suum by bacterial infection. To explore the usefulness of its activity as an antibiotic,...
Effect of Dietary Phytase Transgenic Corn on Physiological Characteristics and the Fate of Recombinant Plant DNA in Laying Hens
Laying Hens Blood Parameters Nutrient Digestibility Recombinant DNA
2016/5/16
The study aimed to evaluate the potential effects of feeding with phytase transgenic corn (PTC) on organ weight, serum biochemical parameters and nutrient digestibility, and to determine the fate of t...
Mapping of QTLs Controlling Carbon Isotope Discrimination in the Photosynthetic System using Recombinant Inbred Lines Derived from a Cross between Two Different Rice (Oryza sativa L.) Cultivars
Carbon isotope discrimination (Δ) Photosynthesis Quantitative trait locus (QTL) Rice (Oryza sativa L.)
2009/7/24
Carbon isotope discrimination (Δ) occuring in the process of photosynthesis, shows variation among rice (Oryza sativa L.) cultivars. Elucidation of specific traits associated with the extent of this d...
Genotypic Differences in Dry Matter Accumulation, Nitrogen Use Efficiency and Harvest Index in Recombinant Inbred Lines of Rice under Hydroponic Culture
Dry matter accumulation Grain yield Harvest index Nitrogen use efficiency for dry matter accumulation Recombinant inbred line
2009/7/21
To examine the possibility of breeding high-yielding cultivars with high nitrogen use efficiency for dry matter accumulation (NUEd) and to provide simple criteria for the selecting and breeding high-y...
Preparation and Characterization of Monoclonal and Recombinant Antibodies Specific to the Insecticide Malathion
organophosphorus insecticide monoclonal antibody scFv ELISA immunoassay
2009/3/7
When mice were immunized with the conjugate EIT244-KLH containing malathion-hapten with a P-NH-C instead of P-S-C bond, two monoclonal antibodies (mabs) MLT2-23 and MLT40-4 specific to the insecticide...
Development of Chromosomal Segment Substitution Lines from a Backcross Recombinant Inbred Population of Interspecific Rice Cross
DNA marker Dongxiang wild rice chromosomal segment substitution line
2009/1/7
A backcross recombinant inbred line population consisting of 202 lines was developed from Xieqingzao B // Xieqingzao B / Dongxiang wild rice. The population was assayed with DNA markers and phenotyped...
Transgenic Expression of the Recombinant Phytase in Rice（Oryza sativa）
transgenic rice recombinant phytase inorganic phosphorus content nutritional qualityRice
2009/1/7
In most of the cereal crop, phytic acid is the main storage form of phosphorus, which can decrease the bioavailability of phosphate. Transgenic expression of phytase is regarded as an efficient way to...