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本研究对亲本海7124、中棉所36及47个子代纤维发育5,10和15天的转录组数据分析,鉴定到35420个表达基因,构建了纤维伸长相关动态表达调控网络,其中10366个表达基因在子代中产生新等位基因(ENAs);进一步鉴定到7个时空特异调控表达基因簇,差异表达分析筛选到105个候选基因,包含15个新等位基因。CRISPR-cas9系统敲除GhMAH1使纤维显著变短。本研究为解析陆海群体纤维长度变异...
A pangenome aims to capture the complete genetic diversity within a species and reduce bias in genetic analysis inherent in using a single reference genome. However, the current linear format of most...
近日,上海市农业科学院作物育种栽培研究所水稻中心,利用代谢组学和转录组学联合分析技术,对水稻巨胚高营养形成的分子机制展开研究,揭示了能量代谢,氨基酸代谢和脂质代谢是水稻巨胚发育早期成熟阶段的关键代谢通路,该研究有助于推动高营养水稻品种的育种。相关研究成果在线发表于国际学术期刊《Frontiers in Plant Science》(IF:5.753,二区TOP)上。
该研究以渗入了海岛棉基因组组分的优质种质鲁原343为亲本,构建了包含239个重组自交系的遗传作图群体,利用SLAF标签构建了高密度遗传图谱,结合7个环境的表型数据,共鉴定104个纤维品质和产量性状QTLs,其中66个QTL共定位于19个QTL簇中。该研究团队通过进一步对鲁原343进行全基因组深度测序,解析鲁原343的遗传组成及其对纤维相关性状的遗传贡献,发现来自G. hirsutum races(...
ATBS1 INTERACTING FACTORs (AIFs)基因是一类bHLH蛋白,在植物生长发育过程中起着重要作用。本研究在亚洲棉、雷蒙德氏棉、陆地棉和海岛棉中分别鉴定得到8, 11, 16和15 AIFs。在该家族基因启动子中发现了多个与生长发育、胁迫和激素相关的启动元件。转录组数据显示该家族基因在棉花的花器官和纤维中高量表达。荧光定量表明该家族基因在纤维起始阶段高量表达。激素处理显示该家...
To understand the genetic basis of nitrogen and phosphorus uptake in the cultivated rice, quantitative trait loci (QTL) analysis for 7 nitrogen and phosphorus uptake-related traits including above-gro...
Fusarium oxysporum Schlecht. f.sp. radicis-lycopersici Jarvis & Schoemaker (FORL) is a saprophytic fungus, responsible for the fusarium crown and root rot disease in tomato (Solanum lycopersicum L.). ...
Laboratory assays were conducted to detect and identify Xanthomonas axonopodis pv. phaseoli on bean seeds collected from naturally contaminated commercial crops. Semi-selective media, viz. Xanthomonas...
Sweet cherry cultivars display a self-incompatibility system that restricts self-fertilization and fertilization between cultivars bearing identical S-alleles. PCR-based S-allele typing system for swe...
Some genetic material was evaluated at Rice Research Institute, Kala Shah Kaku, Lahore during 2002-2004 for its use in hybrid rice technology. One hundred and nine rice genotypes were evaluated for ...
To analyze the genetic control of allelopathy in rice, we examined the quantitative trait loci (QTL) controlling allelopathic effects using a F2 population of the cross between PI312777 and Rexmont, w...
A total of 350 rapid amplified polymorphic DNA (RAPD) primers and 100 simple sequence repeat (SSR) primer pairs were screened to identify polymorphic markers associated with powdery mildew resistance....
Wheat cultivar Jing 411 which is susceptible to powdery mildew, and wheat cultivar Brock and NILs of Jing 411, which are resistant to powdery mildew were analysized for polymophisms using 213 random a...
The callus induction potential of 15 indica rice genotypes from Indonesia was examined in comparison with that of the japonica rice Nipponbare. Callus was induced from embryos of mature seeds and root...
Thirty-nine chromosome segment substitution lines (CSSLs) population derived from a Koshihikari/Kasalath cross was used for quantitative trait locus (QTL) analysis of plant type in rice (Oryza sativa ...

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