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The role of nitric oxide synthase isoforms in aged porcine oocytes
nitric oxide L-NAME aminoguanidine oocyte ageing pig
2015/6/24
In the sphere of reproductive biotechnologies, the demand for sufficient numbers of high-quality oocytes is still increasing. In some cases, this obstacle is overcome by in vitro prolonged cultivation...
Effects of cycloheximide or 6-dimethyl aminopurine on the parthenogenetic activation of pig oocytes using pulsatile treatment with nitric oxide donor
activation nitric oxide oocyte
2015/6/8
Pig oocytes matured in vitro were parthenogenetically activated using nitric oxide donor SNAP (2mM). Continuous treatment successfully activated the oocytes only after more than 12 hours of exposure. ...
Production of Boer goat (Capra hircus) by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes
goat fibroblast clone
2015/6/8
The present study reports a birth of goat clone produced by nuclear transfer of cultured and cryopreserved fibroblast cells into slaughterhouse-derived oocytes. The donors of somatic cells were fibrob...
Porcine oocytes matured in vitro develop in various ways if they are further cultivated. In our studies these oocytes were cultivated for 1 to 5 days (in vitro aging). During the 1st day of aging, mos...
Effects of genistein and genistin on in vitro maturation of pig oocytes
phytoestrogen isoflavone oocyte maturation pig estrogenic effect tyrosine protein kinase
2015/6/5
The objective of the study was to verify the hypothesis that GEN (genistein – phytoestrogen and an inhibitor of tyrosine protein kinase – TPK) effects on pig oocyte maturation and cumular cell expansi...
Parthenogenetic development of rabbit oocytes after electrical stimulation
rabbit oocytes electric pulses in vitro culture
2015/6/2
The aim of this study was to determine the effect of electric pulses on the structural and functional condition of rabbit oocytes. The New Zealand White female rabbits at 3–5 months of age and at 3–4 ...
In vitro maturation and degeneration of domestic cat oocytes collected from ovaries stored at various temperatures
IVM feline COC apoptosis necrosis
2015/4/1
Cat oocytes have the unique ability to mature in vitro after temporary storage at 4°C which can provide opportunities to rescue oocytes from the ovaries of endangered felids after sudden death or medi...
Effect of Dipeptides on In vitro Maturation, Fertilization and Subsequent Embryonic Development of Porcine Oocytes
Ammonia Accumulation Dipeptides Incorporation and Oxidation Porcine Embryos
2016/5/11
The effects of amino acids and dipeptides on in vitro production of porcine embryos and accumulation of ammonia in culture medium during developmental stages were examined in this study. The maturatio...
Effect of the Addition of β-Hydroxybutyrate to Chemically Defined Maturation Medium on the Nuclear Maturation, Sperm Penetration and Embryonic Development of Porcine Oocytes In vitro
Porcine oocytes β-Hydroxybutyrate Nuclear Maturation Embryonic Development
2016/5/5
We investigated the effects of various concentrations of β-hydroxybutyrate (BHB, 0, 0.1, 1 and 10 mM), a ketone body, added to chemically-defined maturation medium with or without energy substrates (g...
Identification of Apoptotic Oocytes in the Developing Ovary of Embryonic and Post-hatched Chicks in Japanese Quail (Coturnix japonica)
oocytes apoptosis development of ovary TUNEL Japanese quail
2009/7/31
The aim of this study was to examine whether apoptosis of oocytes occurs and their frequency changes during the ovarian development of embryo and post-hatched chicks of Japanese quail. The ovaries of ...
Effects of Sperm Pretreatments and In vitro Culture Systems on Development of In vitro Fertilized Embryos Derived from Prepubertal Boer Goat Oocytes in China
Embryo Culture In vitro Fertilization Oocyte Prepubertal Goat Sperm Pretreatment
2016/4/29
Use of oocytes from prepubertal animals for in vitro embryo production holds potential application for reducing generation intervals and increasing genetic progress through embryo transfer. The object...
Improvement of developmental competence of aged porcine oocytes by means of the synergistic effect of insulin-like growth factor-1 (IGF-1) and epidermal growth factor (EGF)
epidermal growth factor insulin-like growth factor 1 oocyte ageing pig
2015/6/1
There is a constant increase in demand for large numbers of high-quality oocytes of domestic mammals for various reproductive biotechnologies. Prolonged in vitro culture represents one of the many pos...