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Purification and quantification of lactoperoxidase in human milk with use of immunoadsorbents with antibodies against recombinant human lactoperoxidase
Lactoperoxidase myeloperoxidase SDS-PAGE
2016/6/13
Two heme-containing peroxidases, secretory lactoperoxidase
and leukocyte-derived myeloperoxidase, which play
host defense roles through antimicrobial activity, were previously
identified in human c...
High-level Expression and Purification of Active Human FGF-2 in Escherichia coli by Codon and Culture Condition Optimization
Fibroblast Growth Factor 2 Codon Protein E. coli
2016/1/26
Background: Basic fibroblast growth factor (bFGF) is a member of a highly conserved superfamily of proteins that are involved in cell proliferation, differentiation, and migration.
Objectives: The ob...
Expression and Purification of the Recombinant Cytochrome P450 CYP141 Protein of Mycobacterium Tuberculosis as a Diagnostic Tool and Vaccine Production
Gene Expression Cytochrome P450 Enzyme System Mycobacterium Tuberculosis
2015/11/6
Background: Tuberculosis (TB) is regarded as a health problem worldwide, particularly in developing countries. Mycobacterium tuberculosis (M. tuberculosis) is the cause of this disease. Approximately ...
Expression and Purification of Recombinant Mycobacterium Tuberculosis (TB) Antigens, ESAT-6, CFP-10 and ESAT- 6/CFP-10 and Their Diagnosis Potential for Detection of TB Patients
Mycobacterium tuberculosis ELISPOT CFP-10 ESAT-6 Tuberculosis Diagnosis
2015/9/23
Background: One of the most widely used methods to detect tuberculosis (TB) infection is the tuberculin skin test (TST). The completion of Mycobacterium tuberculosis (M. tuberculosis) genome sequence ...
Purification and evaluation of somatic, excretory-secretory and Cysteine proteinase antigens of Fasciola Hepatica using IgG-ELISA in diagnosing Fascioliasis
Purification evaluation of somatic excretory-secretory Cysteine proteinase antigens of Fasciola Hepatica IgG-ELISA diagnosing Fascioliasis
2010/1/5
Fasciolosis, or liver fluke disease, caused by parasites of the genus Fasciola is emerging as an important disease in man and animals, in the world and Iran, particularly in nortern parts. The economi...
DOMESTIC WASTEWATER PURIFICATION IN UPFLOW BIOFILM SYSTEM WITH DIFFUSED AERATION
Wastewater purifiction Upflow microbial film System BOD-COD TSS removal
2010/1/4
The objective of this research was to conduct a bench scale study of fixed activated sludge treating domestic sewage. Two different units employing diffused aeration with plastic and aluminum media we...
ISOLATION AND PURIFICATION OF LECTIN FROM IRANIAN RICINUS COMMUNIS SEEDS
Iranian castor bean lectin Column chromatography
2009/12/31
Iranian castor bean lectin was isolated and purified by several column chromatography methods. Then, the sugar and amino acid content of the purified lectin was identified. Castor bean lectin has some...
Isolation,Cloning,Expression and Purification of Recombinant RhD Antigen from Cord Blood
Recombinant RhD antigen Cloning Cord blood
2009/12/7
Background: Rh (Rhesus) is a highly complex blood group system in man deeply rooted in transfusion medicine. Isolation of RhD from cord blod, cloning and expression of recombinant RhD antigen in bacte...
Development of a microfluidic module for DNA purification from bacterial lysates via phenol extraction
DNA Purification Phenol Extraction Multi-Phase Microfluidics
2009/12/24
In this work microfluidic platforms designed to miniaturize the phenol extraction
technique commonly used to purify DNA from cell lysates have been developed.
First, a long serpentine device was use...
Isolation and purification of total RNA from Streptococcus mutans in suspension cultures and biofilms
Dental plaque Streptococcus mutans Polymerase chain reaction RNA Polysaccharides
2009/10/16
The presence of extracellular polysaccharides matrix makes extraction and purification of RNA from Streptococcus mutans within biofilms challenging. In this study, several approaches to purify RNA ext...
Purification of Casein Kinase II From Lung Tissue and Kinetic Evaluations With Polyamines and Myc-Oncoprotein
Casein kinase II olyamines yc ell proliferation abbreviations KII-casein kinase II myc-phosphorylated myc oncoprotein Pmax-phosphorylated max protein
2009/7/2
The overall goal of this study was to seek molecular linkages between polyamines, casein kinase II, and the myc oncoprotein by using a cell-free, highly purified enzyme kinetic analysis. Casein kinase...
Purification and Characterization of Carbonic Anhydrase from Human Erythrocyte Plasma Membrane
Carbonic anhydrase Plasma membrane sulfanilamide KSCN and NaN3
2009/6/29
Carbonic anhydrase is the basic enzyme in inhalation function. Untill now no research had been done to determine whether CA is in the human erythrocyte plasma membrane or not. Carbonic anhydrase (CA) ...
Production and Purification of Monoclonal Antibodies to Human Interleukin-2 and Their Use for Immunoaffinity Purification of Recombinant Human Interleukin-2
Monoclonal antibody human interleukin-2 immunoaffinity purification
2009/6/26
Monoclonal antibodies (mAbs) are a powerful immunochemical tool. Today, the availability of mAbs with desired specificity enables ligand separation based upon the immunoaffinity technique. By using th...
Partial Purification of Topoisomerase I from Mycobacterium phlei
Mycobacterium phlei DNA topoisomerase type I purification molecular properties
2009/6/16
Aim: DNA topoisomerases control several cellular activities by catalyzing the relaxation of superhelices, which are produced during the replication of DNA. Investigation of the inhibitory action of th...
Expression, Purification and Characterization of a Recombinant Plasmodium Vivax Thrombospondin Related Adhesive Protein (PvTRAP)
thrombospondin-related adhesive protein (TRAP) plasmodium vivax malaria
2009/4/20
Thrombospondin Related Adhesive Protein (TRAP) is a transmembrane parasite molecule responsible in sporozoite-host interactions. This molecule is one of the most promising vaccine candidates against t...