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High-level Expression and Purification of Active Human FGF-2 in Escherichia coli by Codon and Culture Condition Optimization
Fibroblast Growth Factor 2 Codon Protein E. coli
2016/1/26
Background: Basic fibroblast growth factor (bFGF) is a member of a highly conserved superfamily of proteins that are involved in cell proliferation, differentiation, and migration.
Objectives: The ob...
Expression and Purification of the Recombinant Cytochrome P450 CYP141 Protein of Mycobacterium Tuberculosis as a Diagnostic Tool and Vaccine Production
Gene Expression Cytochrome P450 Enzyme System Mycobacterium Tuberculosis
2015/11/6
Background: Tuberculosis (TB) is regarded as a health problem worldwide, particularly in developing countries. Mycobacterium tuberculosis (M. tuberculosis) is the cause of this disease. Approximately ...
Expression and Purification of Recombinant Mycobacterium Tuberculosis (TB) Antigens, ESAT-6, CFP-10 and ESAT- 6/CFP-10 and Their Diagnosis Potential for Detection of TB Patients
Mycobacterium tuberculosis ELISPOT CFP-10 ESAT-6 Tuberculosis Diagnosis
2015/9/23
Background: One of the most widely used methods to detect tuberculosis (TB) infection is the tuberculin skin test (TST). The completion of Mycobacterium tuberculosis (M. tuberculosis) genome sequence ...
Purification and evaluation of somatic, excretory-secretory and Cysteine proteinase antigens of Fasciola Hepatica using IgG-ELISA in diagnosing Fascioliasis
Purification evaluation of somatic excretory-secretory Cysteine proteinase antigens of Fasciola Hepatica IgG-ELISA diagnosing Fascioliasis
2010/1/5
Fasciolosis, or liver fluke disease, caused by parasites of the genus Fasciola is emerging as an important disease in man and animals, in the world and Iran, particularly in nortern parts. The economi...
DOMESTIC WASTEWATER PURIFICATION IN UPFLOW BIOFILM SYSTEM WITH DIFFUSED AERATION
Wastewater purifiction Upflow microbial film System BOD-COD TSS removal
2010/1/4
The objective of this research was to conduct a bench scale study of fixed activated sludge treating domestic sewage. Two different units employing diffused aeration with plastic and aluminum media we...
Purification and Characterization of Carbonic Anhydrase from Human Erythrocyte Plasma Membrane
Carbonic anhydrase Plasma membrane sulfanilamide KSCN and NaN3
2009/6/29
Carbonic anhydrase is the basic enzyme in inhalation function. Untill now no research had been done to determine whether CA is in the human erythrocyte plasma membrane or not. Carbonic anhydrase (CA) ...
Production and Purification of Monoclonal Antibodies to Human Interleukin-2 and Their Use for Immunoaffinity Purification of Recombinant Human Interleukin-2
Monoclonal antibody human interleukin-2 immunoaffinity purification
2009/6/26
Monoclonal antibodies (mAbs) are a powerful immunochemical tool. Today, the availability of mAbs with desired specificity enables ligand separation based upon the immunoaffinity technique. By using th...
Partial Purification of Topoisomerase I from Mycobacterium phlei
Mycobacterium phlei DNA topoisomerase type I purification molecular properties
2009/6/16
Aim: DNA topoisomerases control several cellular activities by catalyzing the relaxation of superhelices, which are produced during the replication of DNA. Investigation of the inhibitory action of th...
Purification and characterization of M-177, a 177 kDa allergen, from the house dust mite Dermatophagoides farinae
chromatography Dermatophagoides farinae high-molecular-weight allergen histamine IgE M-177 Mag 3 purification skin test
2010/11/26
A high molecular weight allergen, M-177, was recently discovered in the house dust mite, Dermatophagoides farinae (D. farinae). The aims of this study were to develop a conventional purification proce...
A New Method for the Purification of Cu-Zn Superoxide Dismutase from Human Erythrocytes
Superoxide dismutase (SOD) Purification Erythrocytes SDS-PAGE Cu-Zn Superoxide Iran
2010/2/4
The human erythrocyte is a rich raw material for the purification of Cu-Zn superoxide dismutase (SOD). We applied a simple and rapid procedure for the purification of SOD from human erythrocytes by io...
A SIMPLE AND ECONOMICAL PROCEDURE FOR THE PURIFICATION OF IMMUNOGLOBULIN Y FROM EGG YOLK BY T-GEL CHROMATOGRAPHY
IgY T-Gel Chromatography
2010/4/29
The use of immunoglobulin Y (IgY) instead of polyclonal antibodies prepared in mammals has been reported recently. These antibodies are found to have better properties in terms of specificity and ease...