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Purification and characterization of a sperm motility inhibitor in human seminal plasma
sperm motility inhibitor human seminal plasma
2009/2/23
A sperm motility inhibitor from human seminal plasma was purified and characterized. The purification procedure includes dialysis, ion exchange chromatography on SP-Sephadex C-25 and adsorption chro...
Haptoglobin is a Sertoli cell product in the rat seminiferous epithelium：its purification and regulation
Germ cell testis
2009/2/17
Using multiple HPLC steps, a protein of 67 kDa (estimated by gel permeation HPLC) was purified from Sertoli cell-enriched culture medium that consisted of two dissimilar subunits of 9 (alpha chain...
Partial purification and localization of platelet-activating factor acetylhydrolase from bovine seminal plasma
Partial purification platelet-activating factor bovine seminal plasma
2009/2/17
Platelet-activating factor (PAF) is a potent lipid mediator that is inactivated by platelet-activating factor acetylhydrolase (PAF-AH). Platelet-activating factor bioactivity has been detected in ...
Purification and characterization of a lipophilic factor from testicular macrophages that stimulates testosterone production by Leydig cells
Testis macrophage Leydig cell
2009/2/16
Testicular macrophages have been shown to secrete a factor that stimulates testosterone production by Leydig cells. The purpose of this investigation was to purify and characterize this factor. M...
Purification, cDNA cloning, and developmental changes in the steady-state mRNA level of rat testicular tissue inhibitor of metalloproteases-2(TIMP-2)
Purification cDNA cloning steady-state mRNA level rat
2009/2/16
Using multiple high-performance liquid chromatography (HPLC) steps and high-performance electrophoresis chromatography (HPEC) in conjunction with an [125I]collagen film assay to identify inhibitors ...
Characterization of functional Leydig cells after purification on a continuous gradient of percoll
functional Leydig cells purification continuous gradient of percoll
2009/2/6
Two human chorionic gonadotropin (hCG) responsive cells from rat testicular interstitium were previously isolated on a discontinuous gradient of Percoll. The light cells were non-steroidogenic and...
Characterization and partial purification of the 8-9S androgen receptor from benign prostatic hyperplasia tissues
androgen receptor human prostate benign prostatic hyperplasia
2009/1/5
The present study reports a 3,800-fold purification of the 8-9S androgen-receptor complex from benign prostate hyperplasia (BPH) tissues using differential chromatography. In addition, the BPH androge...
Purification of rat leydig cells：increased yields after unit-gravity sedimentation of collagenase-dispersed interstitial cells
Clusters gap junctions macrophages
2008/12/29
Procedures for purification of Leydig cells have facilitated studies of their regulatory biology. A multistep procedure that includes a filtration with nylon mesh (100-micron pore size) to separate ...