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Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium
18S gene GP60 gene ethidium bromide GelRedTM
2015/4/16
Differentiating between the Cryptosporidium species and their subtypes using only microscopy is impossible. Therefore, molecular tools are indispensable for accurate species and subtype diagnosis. How...
Recovery of Cryptosporidium from spiked water and stool samples measured by PCR and real time PCR
Cryptosporidium parvum oocysts spiked water and stool water filtration
2015/4/16
Cryptosporidium parvum is a common intestinal protozoan parasite infecting humans and a wide range of animals, whose diagnostics present considerable difficulties. These arise from the exceptionally r...
PROGRESS IN THE MOLECULAR METHODS FOR THE DETECTION AND GENETIC CHARACTERIZATION OF CRYPTOSPORIDIUM IN WATER SAMPLES
Cryptosporidium genetic characterization molecular methods
2010/12/22
Cryptosporidium, the protozoan parasite, has several transmission routes,including anthroponotic and zoonotic transmission, as well as the foodborne way, but mainly by water.
Recombinant Cryptosporidium parvum p23 as a Candidate Vaccine for Cryptosporidiosis
Cryptosporidium parvum Expression vector pGEX-5X-2 recombinant protein P23 PCR RT-PCR Western blot Dot Blot
2009/12/14
Background: Cryptosporidium parvum is a ubiquitous protozoan, which develops within the microvillous membrane of enterocytes in a wide variety of vertebrates, including man. Cryptosporidiosis is an im...
Genetic Characterization of Cryptosporidium spp.among Children with Diarrhea in Tehran and Qazvin Provinces,Iran
Cryptosporidum Genotyping SSU-rRNA Children Diarrhea
2009/12/7
Background: Cryptosporidium is an intracellular apicomplexan parasite that infects a wide range of vertebrates including humans. Cryptosporidiosis is a major cause of diarrhea in children with and wit...
Transport of manure-borne Cryptosporidium parvum oocysts through saturatedand unsaturated soil columns
Cryptosporidium parvum soil water transport
2008/11/11
Release of manure-borne Cryptosporidium parvum oocysts is the most probable cause of ground and surface water contamination with this pathogen. Our objective was to assess the effect of water saturati...
Prevalence of Cryptosporidium, Cyclospora cayetanensis and Isospora belli infection among diarrheal patients in South India
Cryptosporidium Cyclospora Isospora
2010/12/10
The emerging protozoan parasites Cryptosporidium, Cyclospora cayetanensis and Isospora belli have altered the etiological spectrum of diarrhea. The progressive decline in CD4 cell count in AIDS patien...
DISTRIBUTION OF CRYPTOSPORIDIUM AND GIARDIA SPP. IN SELECTED SPECIES OF PROTECTED AND GAME MAMMALS FROM NORTH-EASTERN POLAND
Cryptosporidium Giardia reservoir genotype wolf bison beaver
2010/12/23
Cryptosporidium spp. and Giardia spp. are wide-spread pathogens of humans and many species of mammals. The ways of transmission are very complex and diffi cult to defi ne.
SSU- rRNA Gene Analysis of Cryptosporidium spp. in HIV Positive and Negative Patients
Cryptosporidium SSU-rRNA gene HIV Iran
2010/2/8
Cryptosporidium is an apicomplexan parasite of humans and a wild range of domestic as well as wild animals. An 833-bp fragment of the 18S-rRNA gene was used to identify Cryptosporidium spp. recovered ...
Prevalence of Cryptosporidium spp. Infection in Renal Transplant and Hemodialysis Patients
Cryptosporidium spp Renal transplantation Hemodialysis Protozoa Iran
2010/2/4
Transplanted and hemodialysis patients are frequently affected by parasitic diseases such as cryptosporidiosis. Cryptosporidium is a parasite causing self-limited diarrhea and enteritis in healthy ind...
FURTHER EVIDENCE THAT GENOTYPE I AND GENOTYPE II OF CRYPTOSPORIDIUM PARVUM ARE DISTINCT
Cryptosporidium parvum methionine aminopeptidase chaperonin
2010/12/10
Three new genes of Cryptosporidium parvum were cloned, including a gene encoding methionine aminopeptidase, one encoding chaperonin containing T-complex protein 1 delta (TCP-1 delta) and one with unkn...
BIOLOGICAL CHARACTERISATION OF CRYPTOSPORIDIUM PARVUM ISOLATES OF WILDLIFE RODENTS IN POLAND
Cryptosporidium parvum infectivity oocysts measurements
2010/12/24
The study was undertaken to characterise the C. parvum isolates originating from naturally infected woodland and field rodents: Clethrionomys glareolus (CG), Apodemus flavicollis (AF) and Microtus arv...
CALVES AS A POTENTIAL RESERVOIR OF CRYPTOSPORIDIUM PARVUM AND GIARDIA SP.
Cryptosporidium parvum Giardia sp. calves reservoir prevalence
2010/12/27
Studies on cryptosporidiosis and giardiosis were carried out between March and April 1997 on 75 calves from 9 selected farms of Wielkopolska macroregion. Faecal specimens from calves, 3–13 days old, w...