搜索结果: 1-3 共查到“Direct Sequencing”相关记录3条 . 查询时间(0.109 秒)
General-use polymerase chain reaction primers for amplification and direct sequencing of enolase, a single-copy nuclear gene, from different animal phyla
enolase Metazoa, scnDNA
2015/12/11
The dearth of general-use (i.e. quasi-‘universal’) primer
sets for the amplification of single-copy nuclear DNA
(scnDNA) loci, combined with the availability and ease of
use of mitochondrial (mtDNA...
Analysis of ras Gene Mutation in Human Oral Tumours by Polymerase Chain Reaction and Direct Sequencing
Oral tumour PCR Direct sequencing Tobacco-specific nitrosamines
2009/6/29
Genetic alterations in proto-oncogenes or tumour suppressor genes are believed to be one of the key events in the multistage process of carcinogenesis. Activating point mutations occurring in either o...
16S～23S rDNA间区在链球菌和流感嗜血杆菌分类中的应用Identification of Streptococcus Species and Haemophilus influenzae by Direct Sequencing of PCR Products from 16S~23S rRNA Itergenic Sacer Rgions
16S~23Sr RNA间区 链球菌 流感嗜血杆菌 分类
2007/12/30
摘要利用16S~23S rDNA间区（intergenic spacer regions,ISR）在不同细菌中拷贝数、碱基排列、序列长度及所含tRNA基因种类和数目的差异，对15株链球菌和流感嗜血杆菌进行属、种、型和株系的分类鉴定。在16S rDNA的3′端和23S rDNA的5′端的保守区中合成引物，PCR扩增16S~23S rDNA ISR序列，对多态片段切胶纯化直接测序。在GenBank上查...