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Development of an Indirect Competitive Enzyme-linked Immunosorbent Assays Method Based on Immunomagetic-bead for Analyzing Listeria monocytogenes in Food
Indirect Competitive Enzyme-linked Immunosorbent Assays Method
2016/6/2
Listeria monocytogenes is an emerging bacterial food borne pathogen, which could survive common stress levels and often cause listeriosis. To control its infection, it is necessary to establish a sens...
Early Steps of Supported Bilayer Formation Probed by Single Vesicle Fluorescence Assays
Adsorption Diffusion Lipid Bilayers Lipid Bilayers Liposomes Membrane Fluidity Membrane Fusion Membranes,Artificial Microscopy,Fluorescence,Multiphoton Motion Phosphatidylcholines Phosphatidylserines Xanthenes
2016/5/24
We have developed a single vesicle assay to study the mechanisms of supported bilayer formation. Fluorescently labeled, unilamellar vesicles (30-100 nm diameter) were first adsorbed to a quartz surfac...
Food safety and label claims for hazelnut allergy traces:evaluation of two PCR assays
amplification declaration food allergy hazelnut
2015/10/9
The molecular techniques (C-PCR, RT-PCR) in the detection and quantification of allergic substances of hazelnut in various categories of food commodities, e.g. breakfast cereals, chocolates and biscui...
Feed input and excreta collection time in metabolisable energy assays for ducks
metabolisable energy bioassay method force-feeding
2015/6/4
Three experiments were conducted to determine the optimal feed input and excreta collection time by a bioassay of dietary true metabolisable energy (TME) for ducks. In experiment 1 and experiment 2, t...
Development of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortus
Chlamydophila abortus internal control PCR
2015/5/25
Therefore, culture-independent detection methods such as the polymerase chain reaction
are increasingly important and needed. The aim of this study was to develop a polymerase chain reaction assay
...
Development of Polymerase Chain Reaction assays with host-specific internal controls for Chlamydophila abortus
Chlamydophila abortus internal control PCR
2015/4/30
Chlamydophila abortus (C. abortus) is one of the most important infectious agents causing abortion in ruminants. The bacterium is obligately intracellular, cannot grow on agar, but it needs cell cultu...
Demonstration of the effect of epidermal growth factor on ram sperm parameters using two fluorescent assays
viability motility membrane integrity
2015/4/7
ABSTRACT: The goal of this study was to examine the effect of epidermal growth factor (EGF) on sperm viability using two fluorescent techniques and to analyze the obtained results in relation to sperm...
Mixture Models for Single Cell Assays with Applications to Vaccine Studies
Mixture Models Single Cell Assays Applications to Vaccine Studies
2012/9/18
Blood and tissue are composed of many functionally distinct cell subsets. In immunological studies, these can only be measured accurately using single-cell assays. The characterization of these small ...
A Comparative Study on Sensitivity and Specificity of Conventional and Unconventional IgG and IgM Assays for Diagnosis of Toxoplasmosis
Toxoplasmosis Sensitivity Specificity IgG IgM
2009/12/18
Background: Toxoplasmosis is a zoonotic disease with widespread distribution throughout the world. It is caused by the protozoan parasite Toxoplasma gondii. As laboratory diagnosis of toxoplasmos...
An apparatus for performing filtration assays in hyperbaric atmospheres
filtration assay method
2009/7/16
A detailed understanding of the effects of diving gases on the central nervous system will require extensive neurochemical studies. One standard tool of the neurochemist is the filtration assay used t...
Analysis of Borrelia Burgdorferi Antibody Seroprevalence in Patients with Behçet´s Disease Using 3 Different Serological Assays
Behç et´ s disease seroprevalence B.Burgdorferi
2009/6/23
Behçet´s disease is a chronic multisystemic inflammatory vasculitis of unknown etiology. Various infectious agents have been implicated as potential triggering factors in the pathogenesis...
Enzyme-Linked Immunosorbent Assays with High Sensitivity for Antigen-Specific and Total Murine IgE: A Useful Tool for the Study of Allergies in Mouse Models
allergen-specific IgE blocking reagent enzyme-linked immunosorbent assay
2010/11/16
Enhancer solutions are effective in improving ELISAs for total and antigen-specific murine IgE. Selection of blocking reagents was important to decrease unwanted enhancement of background signals and ...
Evaluation of Ribavirin Genotoxicity with Sister Chromatid Exchange and Micronuclei Assays in Humans
Ribavirin genotoxic effect sister chromatid exchange micronuclei formation
2009/5/20
Aim: The main objective of this study was to assess the genotoxicity of ribavirin in 15 patients who were suffering from Crimean-Congo haemorrhagic fever and treated with the therapy dose of the ant...
Detection Assays of Norovirus Genome Using LightCycler®
LightCycler® PCR equipment Norovirus One-Step Real-Time PCR assay
2009/4/8
In 2003, the Ministry of Health, Labour and Welfare in Japan presented the official assay using ABI PRISM 7000® for the detection of Norovirus (NV) genogroups I (GI) and II (GII). We have recently...
A New Oxidase-Peroxidase Kit for Ethanol Assays in Alcoholic Beverages
ethanol enzymatic analysis alcoholic beverages
2009/3/20
The paper presents analytical parameters of an enzymatic method for alcohol assay in alcoholic beverages by the use of a new kit based on alcohol oxidase and peroxidase catalyzed reactions (AOP). Sens...