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生命活动往往是多种细胞协同参与、时空高度调控的复杂过程。因此，对活体中的特定细胞及特定生命分子进行原位的标记、操纵及分析，是研究现代生物学问题的关键途径。基因工程方法（如融合荧光蛋白标签）能够在活细胞中提供精准的标记信号，但其应用范围受限，不适用于一些难以转染的样品（如原代细胞、临床样品等）。而近年来发展起来的生物正交反应能通过化学手段在生命活体中进行原位研究，为生物学研究提供了简单高效的工具。特...
线粒体作为细胞的能量工厂在多个信号通路以及多种疾病的发生发展中扮演了相当重要的角色。对线粒体蛋白质组进行多维度的解析能够使我们更深入地了解它的功能。然而，现有的线粒体蛋白质组学技术存在相应的缺陷。其中，通过密度梯度离心得到的线粒体容易被其他细胞器污染，且对分离后的线粒体进行蛋白质组学分析不能够反映活细胞内的真实情况。酶促邻近标记以及亚细胞定位的活性化学分子标记技术常用于活细胞中的线粒体蛋白质组学分...
探讨慢阻肺患者血清白细胞介素-17（IL-17），收费样品受体4（TLR4）的表达水平与第1秒用力呼气体积占预计值百分比（FEV1％pred），改进版英国医学研究委员会呼吸困难问卷（mMRC分级），慢阻肺评估测试（CAT）评分的相关性。方法：采用 ELISA法检测三组[慢阻肺组，健康吸烟对照（C1）组，健康不吸烟对照（C2）组]血清IL-17，TLR4水平（每组80例），分析其与FEV1％pred...
探索扶芳藤总三萜对D-半乳糖诱导的小鼠衰老模型抗氧化作用。方法 取健康清洁级雄性昆明小鼠75只，以皮下注射D-半乳糖(D-galactose)方法建立衰老小鼠模型；并将小鼠以单纯随机抽样法分为阳性药物组、模型组、扶芳藤总三萜高、低剂量组及正常对照组，每组15只，各组每天按预设方案给药连续21 d后，检测小鼠血清、心脏、肝脏及骨骼肌组织总抗氧化能力(T-AOC)、过氧化氢酶(CAT)、谷胱甘肽过氧化...
Cat B、p53在脑胶质瘤组织中的表达及临床意义
Cat B p53蛋白 胶质瘤 抑癌基因
2020/7/31
分析Cat B、p53在脑胶质瘤组织中的表达及临床意义。方法:选取我院2012年4月至2017年4月期间存档的54例胶质瘤石蜡标本以及同期存档的54正常脑组织标本作为研究对象。采用免疫组化SP法测量两组样本中Cat B、p53蛋白表达水平的差异，观察Cat B、p53蛋白在不同级别脑胶质瘤组织中的表达，分析其相关性。
The 9 Lives of Bleichenbacher's CAT: New Cache ATtacks on TLS Implementations
TLS Bleichenbacher Side-channels
2018/12/3
Over the last twenty years researchers and implementors had spent a huge amount of effort in developing and deploying numerous mitigation techniques which were supposed to plug all the possible source...
You’ve probably heard about Schrödinger’s cat, which famously is trapped in a box with a mechanism that is activated if a radioactive atom decays, releasing radiation. The act of looking in the b...
The brains of wild cats don’t necessarily respond to the same evolutionary pressures as those of their fellow mammals, humans and primates, indicates a surprising new study led by a Michigan State Uni...
Pectus excavatum in a cat:a case report
cat pectus excavatum external splint surgical correction
2016/8/2
This case report aims to clinically and radiologically describe a case of feline pectus excavatum and to evaluate the treatment results. An 8-month-old female cat weighing 3.2 kg was presented to the ...
We propose a technical reformulation of the measurement problem of quantum mechanics, which is based on the postulate that the final state of a measurement is classical; this accords with experimental...
Characterizing entanglement of an artificial atom and a cavity cat state with Bell’s inequality
Characterizing entanglement cavity cat state Bell’s inequality
2016/1/22
The Schrodinger’s cat thought experiment highlights the counterintuitive concept of entan-glement in macroscopically distinguishable systems. The hallmark of entanglement is the detection of strong co...
Emphysematous lesions in the right cranial lung lobe and torsion of the right medial lung lobe in a British shorthair cat: a case report
bullae lobular emphysema spontaneous pneumothorax
2015/12/17
A three-year-old male British shorthair cat that had exhibited progressive lethargy and intermittent
dyspnoea for 14 days was referred for evaluation of acute respiratory deterioration. Clinical fin...
Necrotising fasciitis,a potential threat following conservative treatment of a leucopenic cat:a case report
cat necrotising fasciitis immunosuppression ESBL MRSH HLAR
2015/8/27
An eight-month-old, not vaccinated, intact male domestic shorthair cat from a multi-cat household was presented at the clinic because of fever, inappetence and listlessness. Although leucopenic, it wa...
为比较凡纳滨对虾(Litopenaeus vannamei)的氧化反应和信号转导相关基因在白斑综合征病
毒(WSSV)感染中的变化情况，采用实时荧光定量 PCR，分析 WSSV 感染凡纳滨对虾 6、12、24、
48、72 h 后，鳃和类淋巴组织中硫氧还原蛋白(TRx)、p38 信号通路(LvP38)、过氧化氢酶(CAT)及过
氧化物酶(POD)在 mRNA 转录水平的变化。结果显示，感染对...
Endoscopy-assisted tracheal reconstruction of post-traumatic obstruction in a cat:a case report
cat dyspnoea endoscopy radiographic findings tracheal stenosis
2015/7/28
A domestic shorthair cat was referred with a history of dyspnoea and lethargy that had arisen gradually within the last few days. The cat had been hit by an automobile 10 days earlier. A thoracic radi...