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Proteomic Analysis of Bovine Pregnancy-specific Serum Proteins by 2D Fluorescence Difference Gel Electrophoresis
2D DIGE CyDye Proteomics Bovine Pregnancy Pregnancy-specific Serum Proteins
2016/5/18
Two dimensional-fluorescence difference gel electrophoresis (2D DIGE) is an emerging technique for comparative proteomics, which improves the reproducibility and reliability of differential protein ex...
O-serogroups, virulence genes of pathogenic Escherichia coli and Pulsed-field gel electrophoresis (PFGE) patterns of O149 isolates from diarrhoeic piglets in Korea
Escherichia coli piglet O-serogroup pathotype virulence gene
2015/4/16
A total of 116 Escherichia (E.) coli isolates isolated from neonatal diarrhoeic piglets were serogrouped and tested for the presence of virulence genes for fimbrial and non-fimbrial adhesins, intimin,...
Intermediately virulent Rhodococcus equi isolates from pigs in Slovenia: discovery of new plasmid types and assessment of genetic diversity by pulsed-field gel electrophoresis
genotyping molecular epidemiology restriction enzyme analysis
2015/4/2
The presence of large plasmids in 30 Rhodococcus equi strains isolated from pig lymph nodes with granulomatous changes was investigated. Plasmid DNAs were isolated and digested with the restriction en...
Molecular analysis of Escherichia coli O157 strains isolated from cattle and pigs by the use of PCR and pulsed-field gel electrophoresis methods
E. coli O157 cattle pigs virulence marker genes RFLP genetic relatedness
2015/1/26
Fourteen Escherichia coli O157 strains isolated from cattle and pigs in Poland and in Germany were investigated, using PCR, for the genetic markers associated with Shiga toxin-producing E. coli (STEC)...
Protein Profiling on Meglumine Antimoniate (Glucantime®) Sensitive and Resistant L. tropica Isolates by 2- Dimentional Gel Electrophoresis: A Preliminary Study
2 Dimentional gel Electrophoresis (2 DE) Protein profiling L.tropica Glucantime Sensitive Iran
2009/12/15
Background: Glucantime® is the first- line drug for the treatment of all forms of leishmaniasis. Unfortunately, the prevalence of parasites becoming resistant to Glucantime® is increasing in s...
Analysis of Methicillin Resistant Staphylococcus aureus Isolates by Polyacrylamide Gel Electrophoresis in an Intensive Care Unit of Übni-Sina Hospital
Methicillin Resistant Staphylococcus aureus (MRSA) N-PAGE Turkey
2009/6/30
This study was designed in order to evaluate the relationship of MRSA isolates obtained from an intensive care unit (ICU) and other wards as well as the epidemiological features and typing methods. Tw...
Mutation Analysis By The Use of Temporal Temperature Gradient Gel Electrophoresis
mutation mutation detection techniques molecular scanning nuclear gene mitochondrial mutations
2009/6/22
Today, technological development continues in the mutational analysis area of molecular medicine. In the past few years, the necessity to screen amplified DNA products to identify mutations or polymor...
Regional differences in luminal fluid polypeptides of the rat testis and epididymis revealed by two-dimensional gel electrophoresis
Regional differences luminal fluid polypeptides rat testis epididymis revealed two-dimensional gel electrophoresis
2009/2/20
Luminal fluid samples were collected by micropuncture of the seminiferous tubule, rete testis, and defined levels of the epididymal tubule. After removal of spermatozoa by centrifugation, the supernat...
Detection of DNA damage in response to cooling injury in equine spermatozoa using single-cell gel electrophoresis
DNA damage equine spermatozoa single-cell gel electrophoresis
2008/12/1
Single-cell gel electrophoresis (SCGE), or comet assay, has the ability to detect damage at the single cell level and has not been reported for equine sperm. The ability to detect nuclear damage at ...
Application of Polyacrylamide Gel Electrophoresis and Capillary Gel Electrophoresis to the Analysis of Lactobacillus delbrueckii Whole-Cell Proteins
Lactobacillus delbrueckii whole-cell proteins capillary gel electrophoresis polyacrylamide gel electrophoresis
2008/3/28
Whole-cell protein profiles of different strains of Lactobacillus delbrueckii isolated from dairy products were studied by capillary gel electrophoresis and PAGE. The high similarity among Lactobacill...
Detection of Adulteration of Buttermilk Powder by Gel Electrophoresis
buttermilk powder fat globule membrane proteins nonfat dry milk whey
2008/3/22
Six commercial samples sold as buttermilk powder were compared with an authentic sample by SDS-PAGE, using either large gels (13.5 x 18 x .3 cm) with a manual system or precast miniature gels (4.3 x 5...
Comparison of Milk Proteins Using Preparative Isoelectric Focusing Followed by Polyacrylamide Gel Electrophoresis
comparison milk proteins preparative isoelectric focusing two-dimensional analysis
2008/3/22
The major proteins in milks from bovine, caprine, porcine, and murine animals and from humans were compared using a two-dimensional analysis method. In the first dimension, proteins were separated by ...
Proteomic Comparison between Japanese Black and Holstein Cattle by Two-dimensional Gel Electrophoresis and Identification of Proteins
Cattle Meat Quality Two-dimensional Gel Electrophoresis LC-MS/MS Proteome
2016/4/25
Differences of meat qualities between Japanese Black and Holstein have been known in Japan, however, the causative proteins and/or the genetic background have been unclear. The aim of this study was t...
Effect of Disodium Fumarate on In vitro Rumen Fermentation of Different Substrates and Rumen Bacterial Communities as Revealed by Denaturing Gradient Gel Electrophoresis Analysis of 16S Ribosomal DNA
Disodium Fumarate In vitro Fermentation Denaturing Gradient Gel Electrophoresis
2016/4/25
Two experiments were conducted to investigate the effects of disodium fumarate on the in vitro rumen fermentation profiles of different substrates and microbial communities. In experiment 1, nine diet...
Correlation Analysis between the Breeding Value of Carcass Traits in Hanwoo (Korean Brown Cattle), Bos Taurus, L. and Spot Intensity on Two-dimensional Gel Electrophoresis
Hanwoo Proteomics Spot Intensity Two-Dimensional Gel Electrophoresis Breeding Value
2016/4/22
In order to investigate the genetic marker associated with economic performance in Hanwoo (Korean Brown Cattle), proteomic approach was used. Breeding values were estimated from performance tested ste...