搜索结果: 16-30 共查到“知识库 酶学”相关记录721条 . 查询时间(6.149 秒)
为研究饲料中添加3种不同的碳水化合物对大黄鱼生长性能、饲料利用以及糖代谢关键酶活性的影响，进行为期8周的生长实验和持续24 h的饥饿实验。以葡萄糖、小麦淀粉和糊精这3种碳水化合物作为糖源，设计3组等氮等脂（48%粗蛋白和12%粗脂肪）的饲料。选用初始体质量为（8.51±0.02）g的大黄鱼450尾，随机分为3组（每组3个重复，每个重复50尾）。养殖实验结束后进行饥饿实验，分别在饥饿实验开始后的0、...
同型半胱氨酸及其代谢酶基因多态性与冠心病的关系
冠心病 同型半胱氨酸 基因多态性
2018/9/19
探讨血浆同型半胱氨酸(Ｈｃｙ) 水平及其代谢酶 ＭＴＨＦＲ Ｃ６７７Ｔ、ＭＴＨＦＲ Ａ１２９８Ｃ、ＭＳ Ａ２７５６Ｇ、
ＭＴＲＲ Ａ６６Ｇ 基因多态性与冠心病的相关性ꎮ 方法 在川东北地区汉族人群中 ２２１ 例冠心病患者(冠心病组)和与
之性别、年龄匹配的 ２１０ 例非冠心病患者(对照组)为研究对象ꎮ 采用 Ｈｃｙ 检测试剂盒(速率法)测定两组患者血
浆 Ｈｃｙ ...
脂肪酸移位酶FAT/CD36与长链脂肪酸的结合机制
脂肪酸移位酶 长链脂肪酸 分子模拟对接
2018/9/19
探讨脂肪酸移位酶FAT/CD36与长链脂肪酸的结合机制。 方法 ELISA分析重组蛋白FAT/CD36与不同种类长链脂肪酸结合强弱机制,分子模拟对接进一步验证其结合机制并分析其可能作用位点。 结果ELISA证明重组蛋白FAT/CD36与不同种类长链脂肪酸均显示特异性结合,结合值大小为油酸＞棕榈酸＞棕榈油酸＞肉豆蔻酸＞硬脂酸。分子模拟对接进一步验证FAT/CD36受体蛋白主要与其中的油酸、棕榈酸及棕...
人冠状动脉粥样硬化病灶内CD40L水平与病灶结构变化的关系
冠状动脉粥样硬化 CD40L 基质金属蛋白酶9 病灶结构
2018/9/20
观察人冠状动脉粥样硬化病灶结构变化、CD40L、基质金属蛋白酶9(MMP-9)蛋白表达水平,探讨CD40/CD40L信号在人冠状动脉粥样硬化发生发展中的作用。 方法 收集不同程度粥样硬化的人冠状动脉血管60例作为实验组,以12例无病变的人冠状动脉为对照组,常规HE染色观察两组人群冠状动脉的组织结构并用图像分析软件检测病灶结构的相关指标,免疫组织化学染色检测CD40L、MMP-9蛋白表达水平,分析C...
探讨冠心病患者血清前蛋白转化酶枯草溶菌素9(PCSK9)与小而密低密度脂蛋白胆固醇(sdLDLC)的关系。 方法 收集2014年3月至2014年12月在山西医科大学第一医院住院且行冠状动脉造影术确诊为冠心病的患者100例作为冠心病组,同期健康体检者67例作为对照组。Lipoprint脂蛋白分析仪检测LDLC颗粒大小、sdLDLC颗粒数及sdLDLC所占LDLC的百分比(简称sdLDLC百分比)；酶...
Dissecting Proton Delocalization in an Enzyme’s Hydrogen Bond Network with Unnatural Amino Acids
Dissecting Proton Delocalization Enzyme’s Hydrogen Bond Network Unnatural Amino Acids
2016/5/25
Extended hydrogen bond networks are a common structural motif of enzymes. A recent analysis proposed quantum delocalization of protons as a feature present in the hydrogen bond network spanning a tria...
Quantum delocalization of protons in the hydrogen-bond network of an enzyme active site
ab initio path integral molecular dynamics enzyme hydrogen bonding nuclear quantum effects proton delocalization
2016/5/25
Enzymes use protein architectures to create highly specialized structural motifs that can greatly enhance the rates of complex chemical transformations. Here, we use experiments, combined with ab init...
Ground-state Proton Transfer Kinetics in Green Fluorescent Protein (GFP)
Mitotic centromere-associated kinase (MCAK/Kif2C) Cellular senescence p53 Human primary cells
2016/5/25
Proton transfer plays an important role in the optical properties of green fluorescent protein (GFP). While much is known about excited-state proton transfer reactions (ESPT) in GFP occurring on ultra...
A conserved water-mediated hydrogen bond network defines bosutinib’s kinase selectivity
Humans Water Quinolines Nitriles Aniline Compounds src-Family Kinases Amino Acid Substitution Conserved Sequence Substrate Specificity Hydrogen Bonding
2016/5/25
Kinase inhibitors are important cancer drugs, but they tend to display limited target specificity, and their target profiles are often challenging to rationalize in terms of molecular mechanism. Here ...
GFP Variants with Alternative β‑Strands and Their Application as Light-driven Protease Sensors:A Tale of Two Tails
Green Fluorescent Proteins Peptide Hydrolases Light
2016/5/25
Green fluorescent protein (GFP) variants that carry one extra strand 10 (s10) were created and characterized, and their possible applications were explored. These proteins can fold with either one or ...
Measuring Electrostatic Fields in Both Hydrogen Bonding and non-Hydrogen Bonding Environments using Carbonyl Vibrational Probes
Ribonucleases Molecular Probes Protein Conformation Vibration Acetophenones Molecular Dynamics Simulation Static Electricity Hydrogen Bonding Solvents
2016/5/25
Vibrational probes can provide a direct readout of the local electrostatic field in complex molecular environments, such as protein binding sites and enzyme active sites. This information provides an ...
A Thermodynamic Framework For Identifying Free Energy Inventories of Enzyme Catalytic Cycles
Steroid Isomerases Catalysis Hydrogen-Ion Concentration Thermodynamics Models, Chemical
2016/5/25
Pauling's suggestion that enzymes are complementary in structure to the activated complexes of the reactions they catalyze has provided the conceptual basis to explain how enzymes obtain their fantast...
Quantitative Dissection of Hydrogen Bond-Mediated Proton Transfer in the Ketosteroid Isomerase Active Site
active site environment computational modeling enzyme catalysis protein electrostatics protein semisynthesis
2016/5/25
Hydrogen bond networks are key elements of protein structure and function but have been challenging to study within the complex protein environment. We have carried out in-depth interrogations of the ...
Site-Specific Measurement of Water Dynamics in the Substrate Pocket of Ketosteroid Isomerase Using Time-Resolved Vibrational Spectroscopy
IL-33 ST2 MWCNTs pulmonary fibrosis nanotoxicology
2016/5/25
Little is known about the reorganization capacity of water molecules at the active sites of enzymes and how this couples to the catalytic reaction. Here, we study the dynamics of water molecules at th...
Structural and Spectroscopic Analysis of the Kinase Inhibitor Bosutinib and an Isomer of Bosutinib Binding to the Abl Tyrosine Kinase Domain
Humans Escherichia coli Benzamides Quinolines Nitriles Aniline Compounds Piperazines Pyrimidines Thiazoles Recombinant Proteins
2016/5/25
Chronic myeloid leukemia (CML) is caused by the kinase activity of the BCR-Abl fusion protein. The Abl inhibitors imatinib, nilotinib and dasatinib are currently used to treat CML, but resistance to t...