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采用高效液相色谱技术研究了苦皮藤素Ⅴ在粘虫Mythimna separata和小地老虎Agrotis ypsilon幼虫中的穿透及代谢。结果表明:苦皮藤素V均不能从粘虫和小地老虎幼虫的体壁穿透到血腔或从血腔穿透到中肠,但很容易从中肠穿透到血腔,且穿透速率无差异;苦皮藤素V在小地老虎幼虫体内的代谢解毒速率远大于其在粘虫幼虫体内的代谢速率,其半衰期分别为5.5和13.1 h。本研究结果表明,苦皮藤素V...
one-step reverse transcription-polymerase chain reaction (RT-PCR) protocol was developed and used for the detection of Cherry leaf roll virus (CLRV) and Strawberry latent ring spot virus (SLRSV). The ...
In model experiments with leaf disks from Brassica napus L. subsp. napus and Thlaspi arvense L. and Sinapis arvensis L. in spring periods (May–June) of 2006–2007 feeding preferences of Phyllotreta her...
The distribution of Plum pox virus (PPV) strains, PPV-D, PPV-M and PPV-Rec, was investigated in the Czech Republic in 2005–2008. Fifty-two to ninety-four samples of flowers or leaves of plum, myrobala...
Tomato yellow leaf curl disease (TYLCD) is one of the most devastating plant diseases in the world. As a result of its continuing rapid spread, it now afflicts more than 30 tomato growing countries in...
The pathogenicity of entomopathogenic fungi, Beauveria bassiana, Metarhizum anisopilae and Lecanicillium lecanii, was evaluated against adults of the olive fly Bactrocera oleae (Gmelin) under laborato...
During 1997–1999, the damage and mortality caused by insect herbivores to pigweed (Chenopodium album) and wild buckwheat (Fallopia convolvulus) were studied in an early fallow field at Prague-Ruzyně. ...
Results of research aiming at monitoring of climate changes impact on plant pathogens distribution such as Zucchini yellow mosaic virus (ZYMV), quarantine Plum pox virus (PPV) and quarantine phytoplas...
Environmental factors influence the growth, survival, dissemination and hence the incidence of Fusarium fungi and the disease severity. The knowledge of the quantitative and qualitative effects of env...
Myrobalan latent ringspot virus (MLRSV) was purified from the extract of infected Chenopodium quinoa Willd., using n-octanol, differential centrifugation, and saccharose gradient centrifugation. Ele...
Disease suppression in Ganoderma-infected oil palm seedlings treated with a conidial suspension of Trichoderma harzianum FA 1132 was tested in plant house conditions to determine the effectiveness of ...
An isolate of Grapevine virus A (GVA) from the Czech Republic was obtained from the grapevine cultivar Müller Thurgau. Symptoms of GVA – Kober stem grooving disease were not observed in the infected...
The warm climate pathogen Macrophomina phaseolina (Tassi) Goid., which causes charcoal disease, has been reported in the Czech Republic since 1999. The aim of our work was to study the incidence of ...
Electron microscopy was used to detect the presence of virus particles or inclusions in growth tips and parenchymatic cells of leaves of plum, apricot and peach trees artificially infected with Plum p...
The percentage of diseased plants of the total plants in the plot was around 10%. When inoculated into potato tuber slices, strains of P. marginalis and P. c. subsp. carotovorum produced soft rot....

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