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Post-calving administration of uterine stimulants does not improve reproductive performance in dairy cows
endometritis oxytocin dinoprost ergometrin reproductive parameters
2015/11/26
The aim of this study was to evaluate the effects of administration of oxytocin, in combination with
dinoprost or ergometrin, after parturition on the occurrence of clinical endometritis and subsequ...
Tissue-specific mRNA expression profiles of GDF9,BMP15,and BMPR1B genes in prolific and non-prolific goat breeds
goat prolificacy ovary RT-PCR gene expression
2015/10/29
The tissue-specific mRNA expression profiles of GDF9, BMP15, and BMPR1B genes in goats were investigated. The mRNA expression of the genes was detected in prolific Jining Grey goats and non-prolific L...
Influence of long-term exposure to lead on its accumulation and elimination from tissues and on selected reproductive parameters in the Prussian carp (Carassius gibelio B.) in pond environment
Pb fish bioaccumulation depuration luteinizing hormone
2015/10/29
The bioaccumulation of lead in selected tissues of Prussian carp kept in pond condition during 12 and 24 months of exposure to different doses (8, 13, 24, and 49 mg/kg) of this metal in feed and its e...
胚胎干细胞显微注射对小鼠和猪胚胎发育影响的研究
胚胎干细胞 嵌合体 显微注射
2015/12/2
旨在为小鼠和猪多能性干细胞嵌合体制作提供参考,探讨胚胎干细胞(Embryonic stem cells或Embryonic germ cells,ES/EG)显微注射及受体胚胎发育时期等对胚胎发育的影响。对小鼠ES细胞和猪EG细胞分别进行研究:(1)将小鼠ES细胞分别注入小鼠桑椹胚和囊胚,比较不同发育时期受体胚胎体外培养的发育率和孵化率,以及注射胚胎和未注射胚胎体外培养的发育率和孵化率。结果表明,...
鸡囊胚细胞DMSO细管冷冻保存技术研究
囊胚细胞 DMSO 细管 冷冻保存
2015/12/2
旨在研究高效鸡囊胚细胞(Blastodermal cells,BCs)冷冻保存技术,为利用BCs保存禽类雌性遗传资源以及胚胎干细胞研究奠定基础。采用解冻后二乙酰荧光素 (Fluorescein diacetate,FDA)染色以及培养24 h后细胞贴壁率双重检测BCs活力方法,比较二甲基亚砜(DMSO)不同浓度、不同冷冻与解冻速率等对BCs的冷冻保存效果。结果表明:(1)DMSO不同浓度,20%组...
Effects of bovine colostrum on performance, survival,and immunoglobulin status of suckling piglets during the first days of life
bovine colostrum supplement growth performance field trial nursery pig survivability
2015/9/15
Supplementation of bovine colostrum (BC) has shown to improve growth performance, intestinal development, and immune response in early-weaned pigs. Little is known about whether BC may have similar ef...
Age and sex-related changes in superoxide dismutase activity in bovine tissues
antioxidative enzymes cows calves bulls age sex
2015/9/15
The influence of age, gender, and type of tissue on superoxide dismutase (SOD) activity in bovine organs and tissues was investigated. The investigated material consisted of fragments of tissues and o...
Intraperitoneal lidocaine hydrochloride for prevention of intraperitoneal adhesions following laparoscopic genitourinary tract surgery in ewes
reproduction endosurgery ovary uterus laparoscopic sheep
2015/8/27
Adhesion formation involving the genitourinary tract is common following laparoscopic procedures. To evaluate the effectiveness of intraperitoneal lidocaine hydrochloride 1% solution for the preventio...
绵羊UCP2基因的克隆及其在脂肪组织中的季节性差异表达
基因表达 脂肪组织 季节性
2015/7/28
旨在研究绵羊UCP2基因的克隆和在脂肪组织中的季节性表达差异,以揭示季节对UCP2基因表达的调控作用。本研究采用RT-PCR和RACE法克隆UCP2 cDNA全长序列。以南非肉用美利奴和山西肉用绵羊为对象,用real-time PCR和免疫组化技术检测UCP2 mRNA及其编码蛋白在冬春和夏秋2个阶段6种脂肪组织(皮下脂肪、肠系膜、大网膜、小网膜、腹膜后脂肪和肾周脂肪)中的差异表达。结果表明,绵羊...
貂源伪狂犬病病毒的分离鉴定及gE基因分子特征
分离鉴定 gE基因 貂
2015/7/28
2014年以来,我国多个毛皮动物养殖场不同日龄的貂出现神经症状、腹泻和急性死亡等,采集昌黎县病死貂的脑组织通过细胞培养、病毒蚀斑纯化、病毒的形态结构观察、动物接种试验分离到1株伪狂犬病病毒,命名为Mink 1。病料接种MDCK细胞能够产生典型的细胞病变,病毒粒子在电镜下呈圆形、有囊膜、直径约为150 nm,病毒悬液接种家兔能引起家兔奇痒、麻痹死亡。利用PCR 方法从病死动物脑组织中扩增PRV gE...
关岭牛MyoDI基因启动子上转录因子结合位点的筛选
启动子 细胞核提取物 生物信息学分析
2015/7/28
本研究旨在筛选出关岭牛MyoDI基因启动子上的转录因子结合位点。根据GenBank已公布的牛MyoDI基因的启动子序列,设计特异性PCR引物,扩增贵州关岭牛MyoDI基因的启动子区,构建重组克隆载体pUCM-T-MyoDI -pro,并对阳性质粒进行测序鉴定。再利用筛选试验和生物信息学分析筛选出关岭牛MyoDI基因启动子上的转录因子结合位点。结果,筛选出关岭牛MyoDI基因启动子上含有的转录因子结...
猪IRS4基因多态性、表达差异及其与脂肪沉积性状的关联分析
多态性 表达差异 脂肪沉积性状
2015/7/28
本研究旨在检测猪IRS4基因多态性及其在17种组织中的表达水平,寻找基因内与脂肪沉积性状QTL效应相关的分子标记。本研究采用比较测序方法搜寻到IRS4基因的3个SNPs,分别是在启动子上的g.96C>G,外显子上的同义突变g.1829T>C和错义突变g.1794T>C(p.Phe432Leu)。RT-PCR检测后发现,IRS4基因在垂体、下丘脑中高度表达,在其他组织中弱表达或不表达。在白色杜洛克×...
本研究对5种常用“持家基因”作为鸡肌肉组织发育早期基因和蛋白检测内参基因的有效性进行了评价,为鸡肌肉发育相关分子检测中内参基因的选择提供科学依据。应用实时荧光定量PCR(RT-qPCR)和Western blot技术,分别检测了鸡12胚龄、17胚龄、1日龄和14日龄胸肌中β肌动蛋白(ACTB)、β微管蛋白(TUBB)、TATA结合蛋白(TBP)、甘油醛-3-磷酸脱氢酶(GAPDH)和热休克蛋白70...
LPS诱导条件下猪小肠上皮细胞TLR4及其信号通路基因表达变化分析
TLR4基因 信号通路 细胞因子
2015/7/28
本试验通过0.1和1 μg•mL-1浓度的LPS诱导处理猪小肠上皮细胞(IPEC-J2),分别在2、4、6 h时利用实时荧光定量PCR方法检测TLR4及其信号通路相关基因(CD14、MyD88、TNF-α、IL-1β和IFN-α)mRNA水平相对表达量,初步探讨猪小肠上皮细胞受到产肠毒素大肠杆菌侵扰发生炎症反应的相关分子反应机理。结果发现,两种浓度的LPS均使得所检测的TLR4及其信号...