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Pig Spermatozoa Defect in Acrosome Formation Caused Poor Motion Parameters and Fertilization Failure through Artificial Insemination and In vitro Fertilization
Boar Sperm Motion Parameters In vitro Fertilization Acrosome Defect
2016/5/17
The selection of morphologically normal spermatozoa is critical to obtain high breeding performances in boar breeding farms and artificial insemination (AI) centers. Parameters for the selection of se...
Effects of Gas Composition in the Modified Atmosphere Packaging on the Shelf-life of Longissimus dorsi of Korean Native Black Pigs-Duroc Crossbred during Refrigerated Storage
Korean Native Black Pigs Crossbred with Duroc Storage Time Meat Quality
2016/5/17
This study was conducted to observe the effects of gas composition in modified atmosphere packaging (MAP) on the shelf-life of Longissimus dorsi of Korean Native Black Pigs-Duroc Crossbred (KNP×D) dur...
Effects of Acanthopanax senticosus Polysaccharide Supplementation on Growth Performance, Immunity, Blood Parameters and Expression of Pro-inflammatory Cytokines Genes in Challenged Weaned Piglets
Herbal Extract Stress Growth Cytokines Metabolism Weaned Pigs
2016/5/17
To investigate the effect of dietary Acanthopanax senticosus polysaccharide (ASPS) on growth performance, immunity, blood parameters and mRNA expression of pro-inflammatory cytokines in immunologicall...
Reproductive Performance of Arabian and Thoroughbred Mares under Subtropical Conditions of Pakistan
Mare Reproductive Performance Conception Rate Pakistan
2016/5/16
Breeding records of 57 Arabian and 66 Thoroughbred mares were analysed to assess their reproductive performance under the subtropical conditions of Pakistan. The Arabian mares showed significantly hig...
Principal Milk Components in Buffalo, Holstein Cross, Indigenous Cattle and Red Chittagong Cattle from Bangladesh
Free Amino Acids Milk Fat Globule Size Fatty Acids Minerals Carbohydrates
2016/5/16
The aim of the present study was to get a total physical and chemical characterization and comparison of the principal components in Bangladeshi buffalo (B), Holstein cross (HX), Indigenous cattle (IC...
Prolificacy and Its Relationship with Age, Body Weight, Parity, Previous Litter Size and Body Linear Type Traits in Meat-type Goats
Goat, Black Bengal Prolificacy Linear Type Traits Litter Size Pregnancy
2016/5/16
Data on age and body weight at breeding, parity, previous litter size, days open and some descriptive body linear traits from 389 meat-type, prolific Black Bengal goats in Tripura State of India, were...
Piglets’ Surface Temperature Change at Different Weights at Birth
Infrared Thermography Piglet Weight Thermal Comfort
2016/5/16
The study was carried out in order to verify the effects of piglets’ weight at birth on their surface temperature change (ST) after birth, and its relationship with ingestion time of colostrum. Piglet...
Production of Mutated Porcine Embryos Using Zinc Finger Nucleases and a Reporter-based Cell Enrichment System
Knockout Pig Reporter Based Enrichment Somatic Cell Nuclear Transfer Zinc Finger Nuclease
2016/5/16
To facilitate the construction of genetically-modified pigs, we produced cloned embryos derived from porcine fibroblasts transfected with a pair of engineered zinc finger nuclease (ZFN) plasmids to cr...
山羊肌内脂肪细胞lncRNA鉴别及特征分析
山羊 转录组 肌内脂肪细胞
2014/12/29
为了探讨长链非编码RNA（Long non-coding RNA，lncRNA）在山羊肌内脂肪细胞分化中的作用。本研究以山羊肌内脂肪细胞成熟前后转录组数据，结合CPC（Coding potential calculator）与CPAT（Coding-potential assessment tool）来预测lncRNA，并对其序列、结构与功能进行了分析。结果表明，通过预测得到1 472个山羊肌内脂...
基因敲除作为一种重要的生物技术，被广泛应用于当今生物学、医学研究中，并逐渐扩展到农业、畜牧业和兽医学等研究领域。与传统的同源重组介导的基因敲除相比，近几年发展起来的几种新技术包括RNA干扰（RNAi）、锌指核酶技术（ZFNs）、转录激活子类似的效应子核酶技术（TALENs）和成簇的规律间隔性短回文重复序列 (CRISPR/Cas）等，能够在复杂的基因组和RNA水平引入精细的改变或基因沉默。这些技术...
旨在探讨牛卵丘卵母细胞复合体(Cumulus-oocyte complex ，COCs)体外成熟过程中生长分化因子9(Growth differentiation factor 9, GDF-9)基因表达和卵丘细胞扩展的关系。运用实时荧光定量PCR技术检测GDF-9基因在牛卵丘卵母细胞复合体体外成熟过程中(0、6、12、18、24 和28 h)的相对表达变化，同时观察了卵丘细胞扩展情况。结果表明,...
在体外培养液中添加亚硒酸钠，研究其对延边奶山羊卵母细胞体外成熟及对核移植重构胚胎后期发育的影响。在卵母细胞体外成熟培养液中添加不同浓度的抗氧化剂亚硒酸钠（SS）（0.0、2.5、5.0、10.0 ng·mL-1），以卵母细胞成熟率、细胞内活性氧(ROS)和谷胱甘肽(GSH)水平为指标鉴定试验组卵母细胞成熟质量；以延边奶山羊乳腺上皮细胞为供核细胞，进行体细胞核移植（SCNT），比较重构胚发育能力，染...
旨在克隆和表达林麝IL-1β，为其用于林麝疾病的防治奠定基础。从林麝外周血单核细胞中提取总RNA，RT-PCR扩增IL-1β(Interleukin-1β)基因。将IL-1β成熟肽编码序列连接到pET32a(+)原核表达载体进行表达，纯化目的蛋白并检测其生物学活性。序列分析表明，林麝IL-1β开放阅读框(ORF)长801 bp，编码266个氨基酸,且在反刍动物中高度保守。IPTG诱导林麝IL-1β...
BMP4诱导鸡胚胎干细胞向雄性生殖细胞分化的研究
鸡胚胎干细胞 雄性生殖细胞 分化 BMP4
2014/1/8
旨在探讨BMP4诱导鸡胚胎干细胞向雄性生殖细胞分化的作用机理。采用单层贴壁培养作为分化模式，分别采用10、20、30和40 ng·mL-1 BMP4浓度诱导雄性鸡胚胎干细胞，通过形态学、荧光定量PCR和免疫细胞化学检测诱导效果。根据细胞形态变化和差异基因表达量变化，确定了BMP4的适宜诱导浓度为40 ng·mL-1。在适宜浓度诱导过程中，诱导4 d类胚体开始出现，6~8 d类胚体逐渐增大，数量增多...