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A survey of attitudes of Turkish veterinary students and educators about animal use in research
experimental animal ethics Turkey
2015/2/5
In this study, veterinary students and veterinary educators from FiratVeterinarySchool,IstanbulVeterinarySchool andSelcukVeterinarySchool were surveyed to evaluate their attitudes to animal use in res...
Impaction of right dorsal colon in the horse: report of 32 cases
horse colic right dorsal colon treatment
2015/1/20
Acute as well as chronic and recurrent colic episodes were caused by right dorsal colon (RDC) impaction in 7.98% of all colic patients. Significantly higher statistical evidence was figured out in huc...
Serosurvey of sheep and goats to Chlamydia psittaci in Slovakia during the years 1996-2000
sheep goats Chlamydia psittaci
2015/1/20
In the Slovak Republic during the years 1996–2000, total 30 278 blood samples from sheep and 4 756 from goats were examined serologically by the method of a complement fixation. The IgG antibodies aga...
Changes in serum concentration of 17 beta-estradiol in female rats during estrous cycle after treatment with atrazine and zearalenone
atrazine zearalenone estrous cycle
2015/1/20
A daily dose of 14 mg atrazine and 2.5 mg zearalenone, given p.o. during 5 days of estrous cycle to female rats, changed their estrous cycle in comparison with control animals. On day –1 of expected e...
Professor Josip Ubl's contribution to the development of veterinary medicine in Croatiap
Josip Ubl Croatia history of veterinary medicine
2015/1/20
Numerous data point to the fact that Czech people played an important role in the cultural development of Croatia. Professor Josip Ubl was one of the many outstanding Czech veterinarians who greatly c...
CHARACTERIZATION of GENES REQUIRED for PREIMPLANTATION EMBRYO DEVELOPMENT
gene knockdown pre-implantation RNA factors
2014/10/21
Preimplantation embryo development in the mouse is a time of rapid cellular morphological and molecular changes leading to embryo implantation for the generation of offspring. The Mager lab studies th...
Polygenic Resistance In The Highly DDT-resistant 91-R Strain Of Drosophila Melanogaster Involves Decreased Penetration, Increased Metabolism And Direct Excretion Of DDT
Resistance Metabolism P-glycoprotein
2014/10/21
Resistance to dichlorodiphenyltrichloroethane (DDT) in the 91-R strain of Drosophila melanogaster is extremely high compared to the susceptible Canton-S strain (>1500 times). Oxidative detoxification ...
Title Implication of ADAM Related Metalloproteases in Equine Laminitis
ADAM MMP equine horse
2014/10/21
Title Implication of ADAM Related Metalloproteases in Equine Laminitis。
利用基因芯片分析鸡法氏囊差异表达基因
鸡 基因表达谱 法氏囊 差异表达基因
2014/9/26
为了解鸡法氏囊发育过程中转录组的变化，应用鸡基因表达谱芯片对从18胚龄、10和30日龄SPF鸡法氏囊组织抽提和纯化的mRNA进行芯片杂交，对差异表达基因进行了GO功能分类和KEGG信号通路分析，并利用荧光定量PCR验证了部分差异表达基因。结果表明，法氏囊总RNA的基因检出率在79%~85%，30日龄雏鸡与18胚龄鸡胚相比、10日龄雏鸡与18胚龄鸡胚相比和30与10日龄雏鸡相比分别筛选到5 043、...
小反刍兽疫病毒H基因DNA疫苗的构建及对小鼠的免疫原性
小反刍兽疫病毒 DNA疫苗 H基因 免疫原性
2014/9/26
旨在构建表达小反刍兽疫病毒(PPRV)H蛋白的核酸疫苗，并评价其对小鼠的免疫原性。利用RT-PCR扩增了PPRV的H基因并将其克隆到pcDNA3.1（+）载体中，构建真核表达质粒pcDNA3.1-H，然后转染鸡胚成纤维（CEF）细胞，采用间接免疫荧光和Western blot检测H蛋白的瞬时表达情况。将重组质粒通过后腿肌肉注射的方式免疫BALB/c小鼠，采用间接ELISA、淋巴细胞增殖试验和细胞因...
西南民族大学畜牧兽医学院专业：预防兽医学
西南民族大学 畜牧兽医学院 预防兽医学
2024/5/15
西南民族大学预防兽医学硕士点于1998年经国务院学位委员会批准建立，1999年招收首届研究生，授位类别为农学硕士学位。2008年被确定为四川省省级重点学科。目前该专业设有四个研究方向：（1）动物病原生物学和快速检测技术研究；（2）动物传染病防治；（3）感染与免疫机制；（4）动物寄生虫病防治。目前该硕士点导师组8人中7人具有博士学位，5位教授，3位副教授，其中享受国务院政府特殊津贴者1人，国家民委领...
猪弓形虫重组MIC3蛋白单克隆抗体的制备及其初步应用
岗地弓形虫 MIC3蛋白 单克隆抗体 胶体金
2013/11/28
为获得针对猪弓形虫MIC3蛋白的单克隆抗体，为进一步研制免疫金标试纸条提供材料，作者以纯化的重组MIC3蛋白免疫BALB/c小鼠，运用杂交瘤技术制备单克隆抗体（McAb）。经ELISA 筛选阳性克隆和亚克隆建株，体内诱生腹水法制备McAb，测定其亚类、效价，进行Western blot分析，并利用胶体金颗粒标记的McAb制备免疫金标试纸条。成功制备了2株稳定分泌抗MIC3蛋白单克隆抗体的杂交瘤细胞...
近期, 中国发生人感染H7N9亚型禽流感病毒重大疫情. 本文对病例性别、年龄、职业及地域分布情况以及疫情传播特性和严重程度, 进行深入分析, 结果显示: (1) 与H5N1禽流感人间病例和2009年甲型H1N1人流感大流行病例的分布截然不同, H7N9早期病例中, 老年人(尤其是男性老年人)显著偏多; (2) 按已报道的方法, 此次疫情已是Ⅲ级(严重)疫情, 将上升为Ⅳ级(非常严重)疫情; (3)...
作者拟制备非泼罗尼纳米乳，并对其理化性质及安全性进行分析。以纳米乳的载药量、稳定性和毒性为考察指标，筛选合适的油相、表面活性剂，再利用伪三元相图选出最佳配方，制备出非泼罗尼纳米乳。用透射电镜观察非泼罗尼纳米乳的形态；用激光粒度分析仪测定其粒径分布范围、多分散系数(PDI)和Zeta电位；通过留样观察和加速试验考察其稳定性；通过皮肤毒性试验和急性毒性试验评估其安全性；通过改良的Franz扩散装置考察...