搜索结果: 16-30 共查到“理学 Escherichia coli”相关记录55条 . 查询时间(0.148 秒)
Nearshore hydrodynamics as loading and forcing factors for Escherichia coli contamination at an embayed beach
Nearshore hydrodynamics loading embayed beach
2014/4/4
Numerical simulations of the transport and fate of Escherichia coli were conducted at Chicago’s 63rd Street
Beach, an embayed beach that had the highest mean E. coli concentration among 23 similar La...
基因工程菌株Escherichia coli C600(pRLK14)
流加培养 基因表达 大肠杆菌 基因工程菌株
2009/11/11
在1L搅拌发酵罐中对含有重组质粒pRLK14的大肠杆 菌进行了培养。结果表明,采用二段培养法,于34℃增殖 殖细菌,在对数增殖后期升温到42℃进行诱导,可以高效 表达基因产品半乳品糖激酶。诱导期,醋酸浓度增长较快模 模拟实验表明,控制醋酸浓度可进一步提高半乳糖激酶产率 。
抗菌肽OH-CATH对大肠杆菌Escherichia coli的作用(英文)
抗菌肽 OH-CATH 大肠杆菌 电镜
2009/6/23
OH-CATH是眼镜王蛇中新发现的cathelicidin家族抗菌肽。它在1% NaCl存在的条件下对多种细菌都有较强的抗菌活性,同时,在高浓度下对人红细胞无溶血活性。OH-CATH是开发新型抗菌药物的优良模板。阐明OH-CATH的作用机理及其对微生物的选择性,对研发以OH-CATH为先导结构的药物研发有十分重要的意义。本文利用扫描电镜以及透射电镜对OH-CATH与革兰氏阴性菌-大肠杆菌ATCC ...
An Experiment with Escherichia Coli T Bacteriophage as Tracer in River Flow Studies
Escherichia Coli T Bacteriophage River Flow Studies
2009/6/18
An Experiment with Escherichia Coli T Bacteriophage as Tracer in River Flow Studies.
Differential afferent sensitivity to mucosal lipopolysaccharide from Salmonella typhimurium and Escherichia coli in the rat jejunum
5-HT afferent nerve enteritis hypersensitivity mesenteric
2014/5/16
Postinfectious irritable bowel syndrome may develop subsequent to acute bacterial enteritis. We therefore hypothesized that intestinal afferents may develop hypersensitivity upon exposure to luminal l...
Sensitivity of Escherichia coli to Seaweed (Ascophyllum nodosum) Phlorotannins and Terrestrial Tannins
Ascophyllum nodosum E. coli O157:H7 Phlorotannin Pure Culture Growth Cattle
2016/4/29
Pure culture experiments were conducted to assess the bacteriostatic and bactericidal effects of phlorotannins (PT) isolated from Ascophyllum nodosum (brown seaweed) on Escherichia coli O157:H7. In Ex...
Effects of Achyranthes Bidentata Polysaccharide on Growth Performance, Immunological, Adrenal, and Somatotropic Responses of Weaned Pigs Challenged with Escherichia coli Lipopolysaccharide
Achyranthes Bidentata Polysaccharide Lipopolysaccharide Inflammatory Response Weaned Pigs
2016/4/28
A study was conducted with 48 weaned barrows (283 d, 8.450.14 kg) to determine the effect of Achyranthes bidentata polysaccharide (ABPS) supplementation on pig performance, immunologic...
Identification and Screening of Gene(s) Related to Susceptibility to Enterotoxigenic Escherichia coli F4ab/ac in Piglets
ETEC F4ab/ac Susceptibility Receptor Muc4 Ep-CAM Piglets
2016/4/27
In 2004, J첩rgensen and coworkers proposed the MUC4 gene as a candidate gene of enterotoxigenic Escherichia coli (ETEC) F4ab/ac receptor in piglets and a mutation of G??C in intron 7 of MUC4 was...
摘要I Na m,除其CI基因是d 857温度敏感突变外,其N基因携有amber无义突变,故在Eseherichia coli A19(met 3 thi, hi,一95, ma一19, rel-1)上不能增殖。Act 857只有cI857温度敏感突变,共N基因是野生型,所以能以E. coli A” 为宿主进行增殖。;La 857和A Nam7只有1个N基因之差。},cI 857在A19及其衍生株...
Escherichia coli gal K和gpt基因注人小鼠受精卵的研究
显微注射 杂交 凝胶电泳
2008/1/21
摘要含有E. coli gaIK和gpt基因的重组DNA pPB22用显微注射法导入昆明白小鼠受精
卵,经发育,最后得到21只成年小鼠。其中1只小鼠的肝脏显示出E. coli gpt酶活性,但未检测出E. coli galK酶活性。印迹分子杂交表明,外源的pPB 22重组DNA已整合到2只小鼠的染色体上,其中包括那只肝脏中显示E. cola gpt酶活性的小鼠。
...
牛肠激酶轻链基因的克隆及其在大肠杆菌中的融合表达Cloning and Fusion Expression of Bovine Enterokinase Light Chain Gene in Escherichia Coli
牛肠激酶轻链 克隆 表达 自催化切割
2008/1/16
摘要为克隆表达牛肠激酶(enterokinase)轻链(EKL)编码基因,以期应用于融合蛋白的切割与纯化,从市售北方黄牛十二指肠组织中提取总RNA,以RT-PCR方法扩增其cDNA片段,将此片段克隆于pUCmT载体中,经过特异性限制性内切酶酶切分析片段正确后,进行全序列分析。结果表明,克隆的cDNA与GenBank上的序列相比完全一致,得到了编码正确的牛肠激酶轻链基因全序列。随后,将目的基因片段插...
大肠杆菌FC40系统静止期突变中的F因子转移The Occurrence of Actual F Factor Transfer during the Stationary-phase Mutation in Escherichia coli FC40
大肠杆菌FC40系统 适应性突变 F因子转移
2007/12/31
摘要本研究采用大肠杆菌GM133 rifr细胞和营养收集细胞HB214 strr进行适应性突变实验。在混合30min和2d 后添加链霉素杀死GM133基因型细胞,继续培养5d后,在选择平板上出现了一定数量的lac+strr基因型回复突变菌落。根据这些突变菌落的数量,估计在lac+突变产生之前,GM133和HB214细胞之间的接合频率分别为0.07%和7.47%。在培养了7d的选择平板上添加含链霉素...
摘要 采用微量热法研究了硝酸镧对Escherichia coli B生长代谢过程的影响, 发现高浓度硝酸镧引起E. coli B热谱图出现异常变化: 生长速率常数k值增大、产热峰显著升高和总发热量异常增加. 当硝酸镧浓度为300和500 mg/L时, 培养物在培养过程的总发热量分别是正常条件下的3.89和2.54倍. 用生物学方法对细胞存活率和生物量进行测定结果表明, 细胞在高浓度硝酸镧条件下增殖...
Escherichia coli核糖体蛋白质突变对lac Z表达的影响
核糖体蛋白质 lac Z 翻译特异性 基因表达
2007/12/5
测定了Escherichia coli P90CF[rec A ara D (lac pro B) F’ lac I, pro A+ proB+]及其依赖链霉素突变体(StrDA)、抗链霉素突变体(StrRA)、和回复突变体的b-半乳糖苷酶活性。发现StrDA的酶活性大大低于野生型的酶活性,而StrRA的酶活性与野生型的接近。不同的回复突变体,酶活性差别很大,最高的与野生型的接近,大多数阶于野生性...
专著信息
书名
Asparaginase display of polypeptides in the periplasm of Escherichia coli: potential rapid pepscan technique for antigen epitope mapping.
语种
英文
撰写或编译
作者
Gaofu Q,Jie L,Rongyue C,Xin Y,Dan M,Jie...