搜索结果: 136-150 共查到“基础医学 DNA”相关记录379条 . 查询时间(0.174 秒)
目的: 探讨HBeAg阴性和HBeAg阳性慢性乙型肝炎(CHB) HBV DNA水平和肝组织炎症损害的关系. 方法: 采用荧光定量聚合酶链反应分别对331例CHB(101例HBeAg阴性和230例HBeAg阳性)患者进行血清HBV DNA定量检测和肝组织活检病理炎症分级, 对比分析结果. 结果: 331例CHB患者血清HBV DNA水平与肝组织炎症活动度及肝纤维化程度之间无明显相关性; 101例H...
乙肝病毒外膜大蛋白检测对于判定HBV DNA复制的意义
乙肝病毒包膜大蛋白 乙肝病毒DNA 乙肝
2009/8/7
目的: 通过检测患者血清乙肝病毒外膜大蛋白(HBV-LP)、HBV DNA以及乙肝病毒标志物(HBV M), 探讨HBV-LP对于反映体内乙肝病毒复制的意义.
方法: 采用荧光定量PCR方法对254份HBV感染血清的HBV DNA进行检测, 并采用酶联免疫吸附试验(ELISA)的方法对HBV-LP、Pre-S1蛋白及HBV M进行检测.
结果: 大蛋白的检出结果与HBV DNA的检出结果无...
用PCA方法选择HBV DNA多聚酶TP区VH抗体
PCA方法 HBVDNA多聚酶 TP区 VH抗体
2009/8/3
目的:用PCA方法选择HBV DNA多聚酶TP区VH抗体.方法:从Yeast Display scFv Antibody Library中提取包含scFv抗体库的质粒pPNL6,扩增VH抗体片段.以HepG2.2.15细胞分泌的HBV DNA为模板,PCR扩增HBV DNA末端蛋白(TP).以TP区作为抗原,用PCA方法进行VH抗体选择.结果:PCR扩增的TP区共540 bp.对HBV DNA多聚...
改良聚合酶链反应检测HBV共价闭合环状DNA
改良聚合酶链反应 HBV共价闭合环状 DNA
2009/8/3
目的:建立一种基于聚合酶链反应(PCR) 的简便快速,具有较高敏感性和特异性的检测乙型肝炎病毒(HBV)共价闭合环状DNA(cccDNA)的方法.方法:分别提取HepG2.2.15细胞内的cccDNA及培养上清中的松驰环DNA(rcDNA)样品,试剂盒纯化;设计2对特异性引物,其扩增区域跨越rcDNA单链区;设计2对非特异性引物,扩增区域位于rcDNA双链区.经单链特异性绿豆芽核酸酶(MBN)分别...
目的: 构建携带人幽门螺杆菌(H pylori)中性粒细胞激活蛋白(HP-NAP)基因(napA)的重组活减毒鼠伤寒沙门菌口服DNA疫苗.方法: 应用基因工程技术扩增全长napA，测序并同源性分析后，将其亚克隆入真核表达载体pIRES，鉴定正确后将重组质粒转化活减毒鼠伤寒沙门菌.结果: 重组质粒经PCR及双酶切，证实成功构建了携带HP-NAP基因的重组真核表达质粒pIRES-napA，后者成功转化...
目的: HCV NS5A病毒蛋白反式激活作用的新的靶基因NS5ATP2及其不同剪接体基因序列的确立、克隆化研究. 方法: 依据我室构建的NS5A反式激活基因差异表达的cDNA消减文库，利用生物信息学技术获得，提取HepG2细胞的总RNA，进行反转录(RT-PCR)，扩增产物与原核表达载体连接，进行测序鉴定.结果: 经测序鉴定成功获得新基因的编码序列，并意外发现了NS5ATP2的不同剪接体，对NS5...
Prevalence of Rheumatoid Factor (RF) and Anti-native-DNA Antibodies (anti-n DNA) in Different Age Subpopulations
Rheumatoid factor anti-native-DNA antibodies elderly chronic illness
2009/7/6
In this study, we have evaluated the prevalence of RF and anti-n-DNA in dif-ferent age subpopulations grouped according to their clinical status. RF and anti-n-DNA were measured in the serum of 51 eld...
The Use of Polypeptide Probes Selected From Artificial Peptide Libraries for the Recognition and Differentiation of DNA Sequences
Phage display mutation detection polypeptide probes
2009/7/1
In the novel approach suggested here, we examined the use of polypeptide probes selected from artificial peptide libraries for the identification of the differences in dsDNA sequences which are potent...
西帕依固龈液对LPS抑制人牙龈成纤维细胞DNA合成和细胞周期改变的影响
西帕依固龈液 内毒素 流式细胞仪
2009/6/25
西帕依固龈液对LPS抑制人牙龈成纤维细胞DNA合成和细胞周期改变的影响。
Immunization of Mice with Plasmid DNA Encoding Hepatitis B Virus Core Antigen (HBcAg)
Hepatitis B virus core antigen DNA immunization
2009/6/25
The aim of this study was to investigate the antibody response following immunization with plasmid vector DNA containing the hepatitis B virus core antigen (HBcAg) gene of a hepatitis B virus (HBV) is...
A Preliminary Study of DNA Damage in Peripheral Lymphocytes from Lung Cancer Patients and Healthy Subjects
Comet assay DNA damage Lung cancer Lymphocyte
2009/6/24
A pilot study was undertaken to investigate the differences in oxidative DNA damage and 2-h DNA repair in peripheral lymphocytes between lung cancer patients and healthy subjects. Twenty-four lung ca...
Half a Century of DNA
DNA Fifty years history
2009/6/23
Fifty years have passed since the discovery of the structure of DNA by James Watson and Francis Crick, but in fact the DNA story actually started in 1869 with the discovery of an acidic substance &Ogr...
Analysis of Cell-Free Fetal DNA from Maternal Plasma and Serum Using a Conventional Multiplex PCR:Factors Influencing Success
Noninvasive prenatal diagnosis maternal plasma maternal serum fetal gender determination conventional multiplex PCR
2009/6/23
Recent technology enables the use of cell-free fetal DNA in maternal plasma and serum for noninvasive prenatal genetic diagnosis. This study was designed to evaluate factors most likely to influence t...
Ethidium Bromide Spot Test Is a Simple Yet Highly Accurate Method in Determining DNA Concentration
DNA concentration spot density analysis ethidium bromide
2009/6/19
A commonly used method to measure the amount of DNA in nucleic acid preparations is to use UV irradiation. However, if the DNA sample contains impurities, measuring UV irradiation is misleading. A qui...