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蚕豆豆类胰岛素基因的cDNA克隆及序列分析cDNA Cloning and Sequence Analysis of Leginsulin Gene in Broad Bean (Vicia faba)
蚕豆 豆类胰岛素基因 cDNA序列 RT-PCR
2007/12/30
摘要为探明豆科植物中豆类胰岛素基因的结构特征与进化关系,在已获得大豆豆类胰岛素基因的基础上,以蚕豆种子胚根mRNA为材料,采用RT-PCR技术,克隆了蚕豆豆类胰岛素基因的cDNA序列,编码的前体多肽包括信号肽、成熟型豆类胰岛素及另一多肽的45个氨基酸残基。DNA序列分析表明,克隆片段与大豆和豌豆的同源性分别为62.5%和58.7%。在氨基酸水平上分别具有44.2%和43.6%的同源性,其中存在着高...
MMS-Induced Cytomixis in Pollen Mother Cells of Broad Bean (Vicia faba L.)
Vicia faba MMS microsporogenesis cytomixis pollen fertility stickiness
2010/3/18
Cytomixis was recorded during microsporogenesis in broad bean (Vicia faba L.), a member of the family Fabaceae. It is an important pulse crop used as vegetable, silage, forage and stock feed. The phen...