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Effects of oocyte collection techniques and maturation media on in vitro maturation and subsequent embryo development in Boer goat
Boer goat collection techniques oocyte maturation ovary medium
2015/6/4
The oocytes (experiment 1) were harvested by one of the four collection techniques (slicing, puncture, aspiration I and aspiration II) and the total number and the number of each grade of oocytes were...
Paradigm of Time-sequence Development of the Intestine of Suckling Piglets with Microarray
Intestinal Development Microarray Gene Expression Profile Network
2016/5/10
The interaction of the genes involved in intestinal development is the molecular basis of the regulatory mechanisms of intestinal development. The objective of this study was to identify the significa...
Melatonin Attenuates Nitric Oxide Induced Oxidative Stress on Viability and Gene Expression in Bovine Oviduct Epithelial Cells, and Subsequently Increases Development of Bovine IVM/IVF Embryos
Melatonin Bovine Oviduct Epithelial Cell Bovine IVM/IVF Embryos Nitric Oxide Antioxidant
2016/5/6
The objective of the present study was to elucidate the fundamental mechanism of bovine oviduct epithelial cell (BOEC) co-culture on developmental capacity of bovine IVM/IVF embryos and to determine w...
Effects of Oviductal Fluid, Culture Media and Zona Pellucida Removal on the Development of Porcine Embryos by Nuclear Transfer
Oviductal Fluid Nuclear Transfer Culture Media Zona Pellucida Porcine
2016/4/29
The aim of this study was to compare the effects of oviductal fluid, porcine zygote medium (PZM)-3, PZM-4 and PZM-5, and modified PZM-5 culture media, and determine the effects of zona pellucida (ZP) ...
Stage-specific Expression of Lanosterol 14-Demethylase in Mouse Oocytes in Relation to Fertilization and Embryo Development Competence
Mouse Oocyte Lanosterol 14 -Demethylase In vitro Fertilization Early Embryo Development
2016/4/29
Follicular fluid meiosis-activating sterol (FF-MAS) has been suggested as a positive factor which could improve the oocyte quality and subsequent embryo development after in vitro fertilization. Howev...
In vitro Development of Interspecies Somatic Cell Nuclear Transfer Embryos Derived from Murine Embryonic Fibroblasts and Bovine Oocytes
Murine Bovine Interspecies Somatic Cell Nuclear Transfer b-Mercaptoethanol Xist
2016/4/28
Interspecies somatic cell nuclear transfer (iSCNT) is a useful method to preserve endangered species and to study the reprogramming event of a nuclear donor cell by the oocyte. Although several studie...
Effects of Cell Status of Bovine Oviduct Epithelial Cell (BOEC) on the Development of Bovine IVM/IVF Embryos and Gene Expression in the BOEC Used or Not Used for the Embryo Culture
Bovine Oviduct Epithelial Cell Bovine IVM/IVF Embryos Growth Factor Gene Apoptosis Gene Antioxidant Gene
2016/4/28
The objective of this study was to investigate the effects of cell status of BOEC on development of bovine IVM/IVF embryos and gene expression in BOEC before or after culturing of embryos. The develop...
Effects of Sperm Membrane Disruption and Electrical Activation of Oocytes on In vitro Development and Transgenesis of Porcine Embryos Produced by Intracytoplasmic Sperm Injection
Intracytoplasmic Sperm Injection GFP Transgenic Pigs
2016/4/27
The intracytoplasmic sperm injection (ICSI) procedure has recently been utilized to produce transgenic animals and may serve as an alternative to the conventional pronuclear microinjection in species ...
Vascular Endothelial Growth Factor Has Beneficial Effect Independent of Serum Components throughout Oocyte Maturation and Early Embryonic Development in Cattle
Bovine Embryo Early Development IVM-IVF-IVC Serum VEGF
2016/4/21
In our previous studies, we demonstrated that Vascular Endothelial Growth Factor (VEGF) enhances bovine oocyte maturation and early embryonic development in serum supplemented media. In this experimen...
The Influence of Microinjection of Foreign Gene into the Pronucleus of Fertilized Egg on the Preimplantation Development, Cell Number and Diameter of Rabbit Embryos
Rabbit Egg Blastocyst Microinjection Cell Number Embryo Diameter
2016/4/20
The aim of this in vitro study was to test the effect of microinjection (Mi) of foreign gene into the rabbit egg pronucleus and epidermal growth factor (EGF) addition on the blastocyst rate, the cell ...
Survival and In Vitro Development of Immature Bovine Oocytes Cryopreserved by Vitrification
Bovine Immature Oocytes Polyvinylpyrrolidone (PVP) Vitrification Blastocyst
2016/4/12
The present study was undertaken to investigate the effects of PVP concentration and exposure temperature to vitrification solution on the post-thaw survival, in vitro maturation and development of im...
Differential Influences in Sizes and Cell Cycle Stages of Donor Blastomeres on the Development of Cloned Rabbit Embryos
Nuclear Transfer Cell Cycle Stage Blastomere Cloned Embryo Rabbit
2016/4/12
Experiments were conducted to evaluate the effect of blastomere diameters and cell cycle stages on the subsequent development of nuclear transplant rabbit embryos (NT-embryos) using nuclei derived fro...
Cytoskeletal Patterns, In Vitro Maturation and Parthenogenetic Development of Rabbit GV Oocytes
Germinal Vesicle Cytoplasmic Microtubule In Vitro Maturation Cytoskeleton Rabbit
2016/4/12
The purposes of this study were to optimize the in vitro maturation (IVM) and culture (IVC) systems of rabbit oocytes. Cytoskeletal structures in the germinal vesicle stage (GV) and during IVM are als...
Biochemical Compositions of Follicular Fluid and the Effects of Culture Conditions on the In Vitro Development of Pig Oocytes
Protein Lipid Cholesterol Sex Hormones IVMFC
2016/4/12
The aims of this study were, firstly, to analyze the biochemical compositions of serum and follicular fluid (FF) from prepubertal gilts after PMSG (1,000 IU) treatment. The concentrations of total pro...