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Three-Phase Partitioning for Purification of Laccase Produced by Coriolopsis trogii under Solid Fermentation
Laccase Produced Coriolopsis trogii
2016/6/2
In order to explore separation and purification methods of Coriolopsis trogii laccase production by solid fermentation, we used a three-phase separation technique and wherein the main factors (ammoniu...
Purification and Characterization of Novel Nucleases from a Thermophilic Fungus
Thermophilic fungi DNase RNase Affinity purification
2014/10/22
A thermophilic fungus was isolated from composted horse manure. The organism was as a Chaetomium sp. by sequencing the highly conserved ITS region of the fungus and comparing to known regions in a gen...
Purification and characterisation of the bacteriocin produced by Lactobacillus plantarum, isolated from Chinese pickle
antibacterial activity identification lactic acid bacteria resistant
2014/10/11
119 strains of lactic acid bacteria from Chinese pickle were tested for production of antimicrobial compounds (bacteriocins). Among them, strain C8 showed strongly antibacterial activity against Staph...
Purification and Characterization of Antioxidant Peptide from Sunflower Protein Hydrolysate
antioxidant peptide sunflower protein hydrolysate purification and characterization
2011/2/24
Sunflower proteins were hydrolyzed with Flavourzyme for the production of antioxidant
peptide. DEAE-Sepharose Fast Flow, Sephadex G-25 gel filtration chromatography
and reversed-phase HPLC were cons...
Purification and Properties of a Collagenolytic Protease Produced by Bacillus cereus MBL13 Strain
Bacillus cereus MBL13 collagenolytic protease type I collagen metalloprotease
2010/10/14
A novel collagenase-producing bacterium has been isolated and identified as Bacillus
cereus MBL13. From the culture supernatant of B. cereus MBL13 grown on bone collagen as
the sole carbon and nitro...
Surfactin – A Review on Biosynthesis, Fermentation, Purification and Applications
biosurfactant surfactin Bacillus subtilis biosynthesis fermentation purification
2010/10/14
Surfactin, a bacterial cyclic lipopeptide, is produced by various strains of Bacillus subtilis
and is primarily recognized as one of the most effective biosurfactants. It has the ability to
reduce s...
Purification and Characterization of a Bacteriocin Produced by Lactobacillus lactis Isolated from Marine Environment
Antagonistic activity bacteriocin indicator organisms lactic acid bacteria
2010/9/30
Bacteriocin producing Lactobacillus lactis strain isolated from marine environment, showed broad range of antibacterial activity against some major food borne pathogens. Maximum bacteriocin production...
Purification and Gene Cloning of Catalase from Staphylococcus warneri ISK-1
catalase Staphylococcus purification
2009/7/8
To investigate the possibility of applying staphylococcal catalase to eliminate H2O2 in lactic acid fermentation, an intracellular catalase from Staphylococcus warneri ISK-1 which exhibited high catal...
Purification and Characterization of Extracellular Cysteine Protease Inhibitor from Chlorella sp.
green algae Chlorella cysteine protease inhibitor
2009/7/8
An extracellular cysteine protease inhibitor (ECPI) from Chlorella sp. was purified to homogeneity by DEAE-Cellulose column chromatography and Sephacryl S-300 column chromatography. The molecular mass...
Purification and Some Properties of Pectinesterase from Fruits of a Miniature-Fruited Red Type Tomato
pectinesterase tomato fruits affinity chromatography
2009/7/8
A pectinesterase (E.C. 3.1.1.11, pectin pectylhydrolase) from the ripening stage of fruits of a miniature-fruited red type tomato (Mini-tomato) was purified 247 fold with yield of 26% by ammonium sulf...
Purification and Characterization of Phospholipase D from Cabbage Leaves
phospholipase D cabbage N-terminal amino acid sequence
2009/7/8
Phospholipase D (PLD) was purified from cabbage leaves. The molecular weight of purified PLD was estimated as approximately 73 and 87 kDa by gel filtration using Superdex 200 HR column and, sodium dod...
Purification and Characterization of Feruloyl Esterase from Aspergillus awamori
Aspergillus awamori feruloyl carbohydrate ester feruloyl esterase
2009/7/7
A. awamori feruloly esterases, termed FAE-a and FAE-b, were purified to homogeneity by ammonium sulfate fractionation, DEAE-Sephadex A-50 column chromatography, DEAE-Toyopearl 650 column chromatograph...
Purification of Endopeptidase in Yam Dioscorea opposita and Its Characterization
yam endopeptidase purification
2009/7/7
An endopeptidase in powdered yam was purified using a combination of anion-exchange and gel-filtration chromatographies. The preparation gave 9.2-fold purification with yield of 19% against the crude ...
Purification and Characterization of Intracellular Cysteine Protease Inhibitor from Chlorella sp.
green algae Chlorella cysteine protease inhibitor
2009/7/7
An intracellular cysteine protease inhibitor (ICPI) from Chlorella sp. was purified to homogeneity judging from polyacrylamide gel electrophoresis. The purified inhibitor had a molecular mass of 410kD...
Purification and Characterization of Phytase from Bran of Triticum aestivum L. cv. Nourin #61
phytase phytic acid wheat bran
2009/7/7
Two phytase isozymes (PHY1 and PHY2) were purified homogeneously from bran of Triticum aestivum L. cv. Nourin #61 by (NH4)2SO4 fractionation, methanol fractionation, Sephacryl S-200 HR gel filtration ...