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Leukocyte and Platelet Rich Plasma (L-PRP) Versus Leukocyte and Platelet Rich Fibrin (L-PRF) For Articular Cartilage Repair of the Knee: A Comparative Evaluation in an Animal Model
Platelet-Rich Plasma Cartilage Knee Joint Dogs Articular Cartilage
2015/11/9
Background: Articular cartilage injuries of the knee are among the most debilitating injuries leading to osteoarthritis due to limited regenerative capability of cartilaginous tissue. The use of plate...
实时荧光定量PCR构建绵羊PrP基因标准品质粒和标准曲线。
本文旨在构建有效抑制朊蛋白(Prion protein,PrP)表达的重组质粒,并以此为工具控制PrP表达,从而探讨PrP对细胞SOD活性的影响。设计并化学合成1对含有发夹结构的寡核苷酸片段(shPrP),退火后与表达载体pG-super(Hairpin siRNA expressing vector)定向连接,构建重组质粒pG-super-shPrP。对重组子进行PCR鉴定,测序正确后,脂质体法...
实时荧光定量PCR构建绵羊PrP基因标准品质粒和标准曲线。
实时荧光定量PCR构建绵羊PrP基因标准品质粒和标准曲线。