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Rice mesophyll protoplasts were isolated with ease and with high yield from expanded 2nd leaves, which were grown on agar plates under 23°C, 16.5 W/m2 for 25 days, by using a new cell wall lytic enzym...
To establish a method for isolation and culture of rice protoplasts, enzyme solution compositions, culture conditions, and the selection of cultivars and tissues as materials were examined. The result...
The present study is intended to analyze the early response of protoplasts isolated from soybean suspension cells to electrofusion. It was observed by light microscopy that protoplasts formed a pearl ...
Protoplasts were enzymatically isolated from soybean suspension cells and were cultured in a liquid medium. The reappearance of cell wall at the surface of naked protoplasts was studied by fluorescenc...
Protoplasts of soybean (Glycine max (L.) Merr.) were isolated from suspension cultures and morphological changes during culture were observed using transmission electron microscopy. The regeneration ...
High yields of viable protoplasts were obtained from leaf mesophyll of 10-day-old peanut seedlings (Arachis hypogaea L. var. Lokal). An enzyme solution containing 1% Cellulase YC, 2% Meicelase P-1, 0....
Preparation methods for scanning electron microscopy (SEM) were studied with petunia and rice protoplasts. Fixation schedules to sustain protoplast size during alcohol dehydration were examined. When ...
Ultrastructural changes in chloroplasts of peanut (Arachis hypogaea L. var. Lokal) seedling mesophyll cells and mesophyll protoplasts treated with electric fields were investigated. Generally, chloro...

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