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An aspartic protease 47 causes quantitative recessive resistance to rice black-streaked dwarf virus disease and southern rice black-streaked dwarf virus disease
aspartic proteinase quantitative trait loci resistance variety RBSDV SRBSDV
2023/4/21
利用RACE技术扩增大豆抗病基因同源cDNA 5′末端序列Amplification of 5′ cDNA End of a Disease Resistance Gene Homolog Using RACE System from Soybean
5′-RACE 抗病基因同源序列 大豆
2007/12/31
摘要利用抗病基因保守序列筛选大豆cDNA文库,获得一抗病基因同源cDNA片段,命名为KR3-1。根据KR3-1设计两个基因特异引物(GSP 和 NGSP),分别与通用引物(UPM)和巢式通用引物(NUP)共同扩增,成功地克隆到了该基因的5′末端序列。该扩增片段长447 bp,与已知序列重叠部分为129 bp。
Abstract:Based on part of a known partial c...