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A SIMPLE METHOD OF IN-VITRO CULTURE OF PLASMODIUM FALCIPARUM IN SCREW-CAPPED VIALS1
In-vitro culture techniques multiplication rates
2009/12/30
In a preliminary study carried out in a simple field laboratory in Bandar-Abbas, southern Iran, five isolates of P. falciparum collected from the patients was cultured in RPMI 1640 medium with human s...
The in Vitro Effect of Plasmodium berghei Antigen Lysate on Natural Killer (NK) Cell Cytotoxic Activity
Natural killer (NK) cell cytotoxicity Plasmodium berghei
2009/7/2
Plasmodium berghei is the rodent equivalent of Plasmodium falciparum which causes cerebral malaria in humans. However, P. berghei does not cause cerebral malaria in BALB/c mice, instead it leads to pa...
Proinflammatory cytokines and haematological indices in HIV seropositive adults with uncomplicated Plasmodium falciparum malaria.
Interleukin-2 Interferon gamma HIV
2010/12/10
Background: A prospective study assessing the levels of Interleukin-2 (IL-2) and gamma interferon (IFN-γ) levels, CD4, CD8 and other haematological parameters in a heterogeneous group of HIV sero-posi...
Riboflavin and thiamin status of Nigerian children with Plasmodium falciparum malaria
Riboflavin thiamin Nigerian children Plasmodium falciparum malaria
2010/12/10
Background: Malaria is a major cause of morbidity and mortality in children in sub-Saharan Africa. Since micronutrients have been implicated in the pathogenicity of Plasmodium falciparum, it is impor...
Evaluation of blood preservation methods in the performance of the WHO in vitro micro-test for Plasmodium falciparum in the field
Drug susceptibility test AnaeroPack Plasmodium falciparum
2010/12/10
In vitro drug susceptibility testing of Plasmodium falciparum must be conducted immediately after collecting a sample of the patient‘s blood; otherwise the parasites may weaken and the culture fail. C...
Analysis of Plasmodium vivax Merozoite Surface Protein-1 Gene Sequences
Malaria Plasmodium vivax Merozoite surface antigen (MSP-1) Belem allel (Bel) Iran
2010/2/3
The emergence of Plasmodium vivax in Dashte Moghan in northwestern Iran has become a major concern for Iranian's health officials. Knowledge of genetic make up of the P. vivax populations in this area...
Genetic Diversity in the Circumsporozoite Protein Gene of Plasmodium falciparum from Major Endemic Regions of Iran
Circumsporozoite protein Plasmodium falciparum Malaria Iran
2010/2/8
Circumsporozoite protein (CSP) is one of the stage specific antigens, which is used for the development of vaccines and to investigate the genetic diversity in Plasmodium falciparum malaria parasite. ...
IN VIVO EVALUATION OF COMBINATION EFFECTS OF CHLOROQUINE WITH CEPHARANTHIN® OR MINOCYCLINE HYDROCHLORIDE AGAINST BLOOD-INDUCED CHLOROQUINE-RESISTANT PLASMODIUM BERGHEI NK 65 INFECTIONS
Plasmodium berghei NK 65 Cepharanthin minocycline
2010/12/10
The combination effects of chloroquine with Cepharanthin® or minocycline hydrochloride were evaluated against a blood-induced infection with chloroquine-resistant P. berghei NK 65 in ICR mice. The...
用本所传代已二十余年、对药物敏感的伯氏鼠疟原虫作种源,每转种一代给小白鼠口服单次量咯萘啶。第1代剂量为8 mg/kg,其后剂量每代增加2 mg/kg。转种至第23代,剂量达2,400mg/kg时,虽部分小鼠死亡,存活小鼠的原虫血症仍不转阴,此时原虫的抗药性为亲代原虫的300倍以上。抗咯萘啶鼠疟原虫(RPB2)对氯喹、喹哌、吡咯喹、M-6407、阿的平与青蒿素等有一定程度的交叉抗药性。用对亲代原虫有...