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Determination of HBV Genotypes among Hbs Ag Positive Blood Donors in Tehran,Iran Using PCR-RFLP
HBV Hbs Ag PCR-RFLP Blood Donors Iran
2009/12/17
Background: Hepatitis B virus (HBV) is one of the major causative agents of acute and chronic liver disease worldwide and is believed to be responsible for a million deaths annually. On the basis of a...
IGF2基因PCR-RFLP多态性与脂肪沉积相关性状的关联分析
IGF2基因 PCR-RFLP 背膘厚 猪
2008/1/30
摘要 胰岛素样生长因子2在胎儿生长发育、肿瘤细胞增殖、肌肉生长等方面具有重要的调控作用,是影响猪瘦肉量的主要候选基因。采用PCR-RFLP技术，分析了IGF2基因第8内含子部分片段在猪资源家系群体中的NciⅠ酶切片段多态性分布。IGF2基因该片段具有两个NciⅠ酶切位点（切点位于第8内含子，分别记录为位点A和位点B），两位点在资源家系中均具有多态性。IGF2基因B位点酶切未突变个体均比
酶切突变...
应用聚合酶链反应(PCR)扩增人类LDL受体基因外显子4-内含子4-外显子5片段,PCR产物为1.55kb,DNA片段经序列鉴定后,进行TaqI酶切位点的RFLP分析。结果显示:中国汉族人群LDL受体基因中存在着TaqⅠ酶切位点多态性; 200个LDL受体等位基因中TaqⅠ酶切位点出现的频率为0.515,该点频率较为适中, 可作为中国汉族人群LDL受体基因的遗传标志来进行家族性高胆固醇血症(FH)...
Determination of HCV Genotypes in Iranian Isolates by PCR-RFLP
Hepatitis C Virus Genotyping PCR RFLP Iran
2010/2/3
Hepatitis C is a major cause of liver related morbidity and mortality worldwide and represents a major public health problem. Depending on genomic organization, the virus is divided into six genotypes...