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Acute effects and long-term sequelae of 1,3-dinitrobenzene on male reproduction in the rat. I. Sperm quality, quantity, and fertilizing ability
long-term sequelae 1,3-dinitrobenzene male reproduction rat
2009/2/23
Groups of eight adult male rats were given a single oral dose of 0 or 48 mg/kg of 1,3-dinitrobenzene and sacrificed at 1, 2, 4, 8, 16, 24, 32, 72, and 175 days posttreatment. The groups killed at 17...
Comparison of the penetration ability of human spermatozoa into bovine cervical mucus and zona-free hamster eggs
penetration ability human spermatozoa bovine cervical mucus zona-free hamster eggs
2009/2/20
In vitro bovine cervical mucus (BCM) penetration tests, sperm penetration assays (SPA) using zona-free hamster eggs, and routine semen analyses were performed on a total of 136 freshly collected sem...
The ability of the rat epididymis to concentrate spermatozoa. Responsiveness to aldosterone
aldosterone epididymal spermatozoa
2009/2/19
Experiments have been performed to determine if aldosterone is involved in the control of water reabsorption from the epididymal lumen in vivo. Micropuncture samples of lumen content were collected ...
Relationship Between Fertilizing Ability and cAMP in Human Spermatozoa
capacitation of human spermatozoa cAMP in human spermatozoa fertilization of zona-free hamster eggs
2009/2/19
The goal of this study was to investigate the relationship between cAMP and the fertilizing ability of human spermatozoa. Levels of cAMP were measured in human spermatozoa during 6-hour in vitro i...
Evidence for a Role of Post-Ovulatory Cumulus Components in Supporting Fertilizing Ability of Hamster Spermatozoa
fertilization in vitro capacitation acrosome reaction egg-cumulus complex golden hamster
2009/2/19
A completely chemically defined culture medium was used to investigate the role of egg-cumulus complex (ECC) components in supporting sperm fertilizing ability. Defined sperm motility-stimulating ...
A Competitive In Vitro Assay of Human Sperm Fertilizing Ability Utilizing Contrasting Fluorescent Sperm Markers
Spermatozoa fertilization sperm motility
2009/2/19
Fluorescein isothiocyanate (FITC) and tetramethyl rhodamine isothiocyanate (TRITC) were evaluated for use as contrasting fluorescent labels on living human spermatozoa. Unlabeled (control), FITC...
The impact of reactive oxygen species on bovine sperm fertilizing ability and oocyte maturation
Spermatozoa fertilization development xanthine oxidase
2009/2/17
The objective of this study was to examine the effects of reactive oxygen species (ROS) on bovine sperm function and on the developmental competence of in vitro-matured bovine oocytes. In a first se...
Maturation of monkey spermatozoa in the epididymis with respect to their ability to undergo the acrosome reaction
Capacitation primate development epididymis macaque
2009/2/16
Spermatozoa obtained along the length of the epididymides from five macaque monkeys (Macaca fascicularis) were examined for their ability to undergo the acrosome reaction under basal conditions and ...
Cryopreservation reduces the ability of equine spermatozoa to attach to oviductal epithelial cells and zonae pellucidae in vitro
equine spermatozoa oviductal epithelial cells zonae pellucidae
2009/2/13
Two bioassays were used to evaluate the interaction of fresh and cryopreserved equine semen with oviductal epithelial cells (OEC) and with the zona pellucida (ZP). Split ejaculates were either store...
Synchronous assessment of sperm motility and fertilizing ability in the hamster following treatment with alpha-chlorohydrin
Synchronous assessment sperm motility fertilizing ability
2009/2/13
To investigate the relationship between sperm motion parameters and fertilizing ability, a model was developed to assess both of these endpoints synchronously using a toxicant that inhibits sperm mo...
Effect of bovine oviductal estrus-associated protein on the ability of sperm to capacitate and fertilize oocytes
Oviduct culture proteinsecretion oviductal fluid
2009/2/12
At estrus, an oviduct-specific, estrus-associated glycoprotein (EAP) of molecular weight 85-95,000, is secreted by the oviductal epithelium and found in cannula-derived bovine oviductal fluid (ODF). T...
The ethane dimethanesulfonate-induced decrease in the fertilizing ability of cauda epididymal sperm is independent of the testis
Epididymis sperm maturation in utero insemination fertilization toxicant
2009/2/12
Several decades ago it was reported that when adult male rats were exposed to a single injection of 50 mg/kg body weight ethane dimethanesulfonate (EDS) and mated with untreated females, average l...
Accessory sperm as an indication of fertilizing ability of rabbit spermatozoa frozen in egg yolk-acetamide with detergent
Accessory sperm fertilizing ability of rabbit spermatozoa
2008/12/26
Many factors besides initial semen quality affect fertilization rates as sperm interact with the environment of the female reproductive tract. One of these factors is sperm transport, which can be eva...
Supplementation of the Thawing Media With Reduced Glutathione Improves Function and the In Vitro Fertilizing Ability of Boar Spermatozoa After Cryopreservation
Pig spermatozoa oxidative stress IVF capacitation status
2008/12/23
In this study, we evaluated the effects of glutathione (L-g-glutamyl-L-cysteinylglycine; GSH) supplementation of the thawing extender on semen parameters to compensate for the decrease in GSH conten...
Does Seminal Plasma PSP-I/PSP-II Spermadhesin Modulate the Ability of Boar Spermatozoa to Penetrate Homologous Oocytes In Vitro?
Seminal plasma preservation sperm viability IVF pig
2008/12/22
Low concentration (0.15 mg per million of spermatozoa) of seminal plasma–derived PSP-I/PSP-II spermadhesin heterodimer is able to preserve the viability of highly extended boar spermatozoa. Whether ...