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A panel of monoclonal antibodies against human sperm
Antisperm antibodies sperm antigens serum immunolocalization
2009/2/17
To help identify and characterize antigens involved in sperm functions and immune infertility, monoclonal antibodies (mAbs) were raised against human sperm antigens. The immunoglobulin G (IgG) fra...
Molecular characterizations of an intraacrosomal antigen defined by HS-33 monoclonal antibody
Sperm antigen anti-sperm antibodies acrosome reaction
2009/2/17
Among the numerous anti-sperm monoclonal antibodies generated in our laboratory, HS-33 was shown to react with a conserved antigen on the acrosome of spermatozoa from human and mouse. By using ind...
Anti-bull sperm monoclonal antibodies:II. Binding changes during capacitation and influence on sperm-zona interactions in vitro
Bull sperm sperm-zona binding capacitation
2009/2/16
Anti-bull sperm monoclonal antibodies (mAbs), generated against intra-acrosomal and surface antigens, were evaluated for their functional significance. In experiment I, the influence of mAbs on the bo...
Anti-bull sperm monoclonal antibodies:I. Identification of major antigenic domains of bull sperm and manifestation of interspecies cross-reactivity
Anti-sperm antibodies antigen localization conserved
2009/2/16
The overall objective of this series of experiments is to generate immunological markers that may elucidate bull sperm surface changes in vitro. Here we report the initial experiments of the study, ...
Use of neonatal tolerization and chemical immunosuppression for the production of monoclonal antibodies to maturation-specific sperm surface molecules
neonatal tolerization chemical immunosuppression monoclonal antibodies
2009/2/7
Mammalian sperm acquire functional maturity as they move from the caput to the cauda epididymidis. Changes occur in the protein/glycoprotein composition of the sperm plasma membrane during this time...
Stage-specific expression of a protein of the acrosome of baboon sperm during spermiogenesis detected with a monoclonal antibody
Spermatogenesis spermiogenesis acrosome monoclonal antibody
2009/2/7
A single monoclonal antibody, BSA4, raised against baboon epididymal sperm was used to study the ontogeny of the baboon sperm acrosome region during testicular spermiogenesis. This antibody is not s...
Preparation and characterization of monoclonal antibodies against sperm-specific lactate dehydrogenase C4
LDH-C4 monoclonal antibodies specificity
2009/2/6
LDH-C4, the testis-specific isozymes of lactate dehydrogenase (LDH), is the predominant LDH isozyme in mammalian spermatozoa. Nine monoclonal antibodies against mouse LDH-C4 have been developed. The...
Monoclonal Antibody From Vasectomized Mouse Identifies a Conserved Testis-Specific Antigen TSA70
Vasectomy testicular auto antigen acrosomal matrix capacitation
2008/12/23
Vasectomy results in the occlusion of testicular outflow, leading to autoimmunity characterized by the production of antisperm antibodies (ASA). Reports on the rise in ASA following vasectomy in sev...
Identification and Characterization of an Antigen Recognized by Monoclonal Antibody TRA 54 in Mouse Epididymis and Vas Deferens
Testis sperm antigens cryptorchidism castration testosterone replacement
2008/12/22
Spermatozoa in testicular fluid are known to have weak forward motility and cannot fertilize eggs. The epididymis is known to participate in sperm maturation leading fertilization, but little is k...
Identification and Characterization of an Antigen Recognized by Monoclonal Antibody TRA 54 in Mouse Epididymis and Vas Deferens
Testis sperm antigens cryptorchidism castration testosterone replacement
2008/12/22
Spermatozoa in testicular fluid are known to have weak forward motility and cannot fertilize eggs. The epididymis is known to participate in sperm maturation leading fertilization, but little is k...
Identification and Characterization of an Antigen Recognized by Monoclonal Antibody TRA 54 in Mouse Epididymis and Vas Deferens
Testis sperm antigens cryptorchidism castration testosterone replacement
2008/12/22
Spermatozoa in testicular fluid are known to have weak forward motility and cannot fertilize eggs. The epididymis is known to participate in sperm maturation leading fertilization, but little is k...
Monoclonal Antibodies to Epididymis-specific Proteins Using Mice Rendered Immune Tolerant to Testicular Proteins
Neonatal tolerization epididymis monoclonal antibodies
2008/12/19
Monoclonal antibodies (mabs) have been used as a powerful tool for identification of newer sperm proteins. However, conventional hybridoma technology rarely provides chance to obtain mabs to epididy...