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Normal semen has up to three polymorphonuclear leukocytes per high power field (HPF). Reduced zinc concentrations (<15 mg/dl), elevated pH (>7.7), and neutrophils (5-10 leukocytes/HPF) in semen can ...
A sensitive radiometric assay was developed for detecting phospholipase A1 and A2 (PLA1 and A2) activity. Four to five times more PLA2 activity was observed in rat testes than PLA1 activity. Two PLA...
PH-20 is a sperm plasma-membrane protein that has been shown to have hyaluronidase activity in several mammalian species including nonhuman primates. In this investigation, the PH-20 protein was c...
To better understand the biology of snow leopard spermatozoa and to facilitate developing assisted reproduction, a series of studies was conducted to: 1) identify the component(s) of complex cultu...
The proteolytic degradation of human seminal fluid proteins at acidic conditions has been investigated. Upon acidification to the pH level of the human vagina, autoproteolysis of most seminal fluid ...
PH-20 is a glycoprotein located on the surface of the sperm plasma membrane and on the inner acrosomal membrane. The best understood function of sperm surface PH-20 is its hyaluronidase activity, ...
The gene for the sperm adhesion molecule 1 (PH-20), SPAM1, has been known to be testis-specific and exclusively haploid expressed. We show that in mice, the 2 common isoforms of the protein (Spam1...
Intracellular pH (pHi) was determined in ejaculated bovine spermatozoa using a ratiometric absorbance technique under various incubation conditions that drastically altered sperm motility. The pHi w...
Previously we demonstrated that the murine sperm adhesion molecule 1 (Spam1 or PH-20) is synthesized by the epididymal epithelium, preferentially in the distal region, and is released into the lumin...
It is well known that the plasma membranes of mammalian spermatozoa undergo extensive remodeling during maturation in the epididymal duct. In this investigation, we have used fluorescence recovery a...
PH-20 is a glycoprotein located on the surface of the sperm plasma membrane and on the inner acrosomal membrane. The best understood function of sperm surface PH-20 is its hyaluronidase activity, wh...
目的 研究不同pH值的精子获能液对精子胞浆内Ca2+水平的影响。 方法 取15例正常生育男性精液,离心所得精子沉淀用不同pH值(4.2,5.2,6.2,7.2,8.2,9.2,10.2,11.2)的精子获能液处理,采用流式细胞仪和激光共聚焦显微镜分别检测精子胞浆内游离钙离子的平均荧光强度值。 结果 流式细胞仪结果显示,与pH7.2(209.94±89.02)相比,pH为6.2时,精子胞浆内钙离子...

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