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Enhanced Nitrogen Fixation Capabilities of Soybean Rhizobia by Inter- and Intra-specific Cell Fusion
(Brady) Rhizobium Cell fusion Nitrogenase Nodulation Protoplast Soybean
2009/7/28
A procedure for protoplast isolation and regeneration in rhizobia has been established. The use of 1% N-laurylsarcosine in pre-washing of the cells facilitated the cellular lysis by incubation for 1 h...