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线粒体DNA在先天性免疫中的作用
线粒体DNA 先天性免疫 感染 炎性疾病
2021/3/5
线粒体是细胞内重要的细胞器,具有多种功能,是细胞的能量工厂。线粒体含有自己的遗传物质线粒体DNA,线粒体DNA因其在氧化磷酸化中的作用而广为人知。近年来,越来越多的研究表明线粒体DNA可作为先天性免疫系统的激动剂,并在病原体感染和炎性疾病的病理发展中起重要作用。线粒体DNA在进入细胞质或细胞外环境后,可以激活多种先天性免疫系统的模式识别受体,从而触发促炎细胞因子分泌和Ⅰ型干扰素反应。因此,本文就线...
本研究计划构建能同时表达牛病毒性腹泻病毒(BVDV)和牛传染性鼻气管炎病毒(IBRV)优势抗原基因的重组质粒并研究其免疫效果,旨在为BVD-IBR二联核酸疫苗的研制提供参考。采用PCR扩增目的基因E0及gD并将其依次连接至真核表达载体pVAX1-IRES中,构建pVAX1-E0-IRES-gD重组真核表达载体;将pVAX1-E0-IRES-gD转染至293T细胞中,间接免疫荧光法检测目的基因在细胞...
经典遗传学认为生物的遗传信息是由基因决定的,DNA序列发生的改变可以传递给后代,而表观遗传修饰则由于细胞重编程作用不能被传递到后代。近年来的研究发现,一些环境因素引起的表观遗传修饰也可以传递给后代,而DNA甲基化是表观遗传修饰的主要机制之一,也是近年来研究跨代遗传的热点。本文对近年来环境因素在哺乳动物世代继承中的DNA甲基化修饰相关研究进行综述,为进一步研究跨代遗传中的表观遗传修饰机制提供参考。
维生素C对藏猪精液4 ℃保存及其精子全基因组DNA甲基化的影响
维生素C 藏猪精液 4 ℃保存 DNA甲基化
2016/5/23
旨在研究维生素C对藏猪精液4 ℃保存效果及其精子全基因组DNA甲基化的影响。用手握法采集健康藏猪精液,在改进的猪精液低温稀释液中添加不同浓度的维生素C(0.0、2.5、5.0、7.5、10.0 g•L-1),4 ℃保存到第5天时,以藏猪精子活力、精子畸形率、精子顶体完整率和质膜完整率为评价指标,探讨维生素C对藏猪精液4 ℃保存的效果;用甲基化荧光定量法检测藏猪新鲜精液组、保存到第5天时...
表达柔嫩艾美耳球虫HMGB1基因质粒DNA的免疫效果分析
高迁移率组蛋白 真核表达 免疫保护
2016/2/24
旨在评价表达柔嫩艾美耳球虫(E.tenella)HMGB1基因质粒DNA免疫对同源株攻虫的免疫保护效果。将EtHMGB1基因插入pcDNA3.1(-)/myc-His A真核表达载体中,并在Hela细胞中进行体外瞬时表达。设计了pcDNA3.1(-)/myc-EtHMGB1免疫组、pcDNA3.1(-)/myc-His A免疫组、重组蛋白质+FCA佐剂免疫组、pcDNA3.1(-)/myc-EtH...
本研究旨在克隆绵羊生长分化因子9(Growth differentiation factor 9,GDF9)基因mRNA、DNA和调控区全序列,并检测其在11个绵羊品种中的遗传多态性,探究GDF9基因与绵羊多羔性状的关系。首先应用RACE和PCR技术克隆GDF9基因全长序列,其次利用SNaPshot分型技术检测11个绵羊品种GDF9基因遗传多态性。结果显示,克隆获得GDF9基因mRNA全长1 85...
Intra-line and inter-line genetic diversity in sire lines of the Old Kladruber horse based on microsatellite analysis of DNA
genetic variability genetic structure conservation microsatellite DNA Kladruber horse line
2015/6/9
The Old Kladruber horse is the oldest original Czech horse breed – gene resource, with pedigree records spanning three centuries. Because the population is closed, there is a concern about the loss of...
Mycoplasma gallisepticum strains with identical random amplified polymorphic DNA (RAPD) patterns in chukar partridges (Alectoris chukar) and broilers: a case report
RAPD Mycoplasma gallisepticum chukar partridge broiler
2015/4/17
We used the random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) technique to discriminate the major emerging poultry pathogen, Mycoplasma gallisepticum (MG), in broiler and chukar pa...
Comparison of techniques for DNA extraction and agarose gel staining of DNA fragments using samples of Cryptosporidium
18S gene GP60 gene ethidium bromide GelRedTM
2015/4/16
Differentiating between the Cryptosporidium species and their subtypes using only microscopy is impossible. Therefore, molecular tools are indispensable for accurate species and subtype diagnosis. How...
DNA integrity in fresh, chilled and frozen-thawed canine spermatozoa
dog DNA fragmentation index sperm chromatin structure assay (SCSA) cryopreservation chilled semen
2015/4/16
Sperm chromatin status in fresh dog semen and the effect of long-term storage of chilled and frozen dog semen on sperm chromatin integrity was assessed by the sperm chromatin structure assay (SCSA). I...
DNA damage of blood lymphocytes and neutrophils in cattle with lymphosarcoma
apoptosis cattle lymphocytes lymphosarcoma neutrophils
2015/4/15
The objective of the present study was to analyze the apoptotic process in peripheral blood mononuclear cells (PBMC) and polymorphonuclear neutrophil leukocytes (PMN) in cows clinically affected with ...
Co-expression of IBV structural proteins and chicken interleukin-2 for DNA immunization
IBV S1 gene IL2 expression
2015/4/2
The S1 gene of IBV and the chicken IL-2 gene were ligated together into a eukaryotic expression vector pCI-neo for co-expression evaluation in vitro. The recombinant plasmids were transfected into Ver...
本研究旨在将ERIC-PCR基因指纹图谱技术应用于兔粪微生物的分析中,建立一种快速分析检测兔肠道菌群变化的方法。通过对PCR反应条件的优化确定适合兔粪微生物的ERIC-PCR反应条件,对比兔粪微生物组和兔粪优势菌的ERIC-PCR DNA指纹图谱差异,从而分析兔粪微生物ERIC指纹图谱的特征。结果建立了适合兔粪样品 ERIC-PCR 分析的反应体系,确定450 bp处条带为双歧杆菌特征条带,1 3...
Methods of mycobacterial DNA isolation from different biological material: a review
Johne’s disease Crohn’s disease zoonoses
2015/3/5
Mycobacteria cause serious infections in animals and human beings. Huge economic losses on farms are caused by selected species of this wide family. A high risk of transmission of infection from anima...
Bull and boar sperm DNA integrity evaluated by sperm chromatin structure assay in the Czech Republic
flow cytometry bull boar fertility
2015/2/5
Analysis of sperm parameters is very important for predicting the outcome of assisted reproductive techniques and is necessary for determination of fertility potential of males tested for artificial i...