搜索结果: 16-30 共查到“知识库 医学微生物学”相关记录540条 . 查询时间(4.681 秒)
Helicobacter pylori In Uninvestigated Dyspepsia In Primary Cares In Abakaliki,Nigeria
H. pylori infection Prevalence Dyspepsia Ulcer
2015/7/29
There is paucity of information on the prevalence of Helicobacter pylori infection in Nigeria. Enzyme-linked immunosorbent assay (ELISA) was used to determine the prevalence of immunoglobulin G (IgG) ...
Spontaneous Bacterial Peritonitis in Subclinical Hypothyroidism
Peritonitis Ascites subclinical Hypothyroidism
2015/7/10
Hypothyroidism is an uncommon cause of ascites. Here we describe a case of a 75 year-old female patient with spontaneous bacterial peritonitis and subclinical hypothyroidism that resolved with thyroid...
The effects of CaMKII signaling on neuronal viability
Calcium/calmodulin-dependent protein kinase II CaMKII Neuron Neurotransmitter Neurotoxicity
2015/5/21
Calcium/calmodulin-dependent protein kinase II (CaMKII) is a critical modulator of synaptic function, plasticity, and learning and memory. In neurons and astrocytes, CaMKII regulates cellular excitabi...
Nf1-DEFICIENT MICE DISPLAY SOCIAL LEARNING DEFICITS THAT ARE RESCUED BY THE DELETION OF PAK1 GENE
Neurofibromatosis type 1 (NF1) social learning p21-activated kinase 1 (PAK1) MAPK prefrontal cortex basolateral amygdala
2015/5/21
Neurofibromatosis type 1 (NF1) is a neurocutaneous disorder that affects roughly 1 in 3500 individuals. In addition to physical features (e.g., neurofibromas), developmental disorders are also common ...
A STUDY OF THE BETA-2 TOXIN GENE AND THE BETA-2 TOXIN IN CLOSTRIDIUM PERFRINGENS STRAINS ISOLATED FROM HUMAN SOURCES
Clostridium perfringens beta-2 toxin
2015/1/19
Clostridium perfringens is an important human pathogen known to cause a range of diseases including diarrhea, necrotizing bowel disease and gas gangrene. Though potentially pathogenic, this microorgan...
Detection of Class I Integrons in Staphylococcus aureus Isolated From Clinical Samples
Staphylococcus aureus Drug Resistance Polymerase Chain Reaction
2015/10/15
Background: Staphylococcus aureus is a major pathogen, causing variety of diseases and death in Iran and in the world. Despite the use of a spectrum of new antibiotics, this organism has caused severe...
Staphylococcal Enterotoxin C in Synovial Fluid of Patients With Rheumatoid Arthritis
Staphylococcal Enterotoxin C Synovial Fluid PCR ELISA Rheumatoid Arthritis
2015/10/15
Background: In the previous studies using the commercial ELISA kit, the existence of staphylococcal superantigens has been reported in synovial fluid of patients with rheumatoid arthritis (RA).
Virulence Gene Profiles of Multidrug-Resistant Pseudomonas aeruginosa Isolated From Iranian Hospital Infections
Pseudomonas aeruginosa Virulence Factors Hospital infections Iran
2015/10/15
Background: The most common hospital-acquired pathogen is Pseudomonas aeruginosa. It is a multidrug resistant bacterium causing systemic infections.
Nasal Carriage and Resistance Pattern of Multidrug Resistant Staphylococcus aureus Among Healthy Children in Kashan,Iran
Staphylococcus aureus Nasal Carriers Multidrug Resistance Healthy Child
2015/10/14
Background: Nasal carriage of Staphylococcus aureus is a substantial source of human infections. Detection and treatment of nasal carriage in children with methicillin-resistant and multidrug resistan...
A Comparative Serological Study of Toxoplasmosis in Pregnant Women by CLIA and ELISA Methods in Chalus City Iran
Toxoplasma Toxoplasmosis in Pregnancy Congenital Infection
2015/10/12
Background:: Toxoplasmosis is the most common disease in humans and animals (zoonosis) caused by the protozoan parasite Toxoplasma gondii. The disease is usually appeared as asymptomatic in immunocomp...
金黄色葡萄球菌N315 T7噬菌体cDNA展示文库的构建
噬菌体展示 cDNA文库 耐甲氧西林金黄色葡萄球菌 N315
2014/7/30
构建MRSA N315菌株的T7噬菌体cDNA展示文库，以筛选和鉴定影响N315 ccr基因表达的调节因子。 方法 采用Tripure试剂提取N315总RNA，逆转录合成双链cDNA，经末端修平、接头连接、酶切后柱洗脱等处理后，连接T7Select 10-3载体，体外包装扩增获得N315 T7噬菌体cDNA展示文库；通过双层平板实验鉴定所建原始文库及扩增文库的库容；PCR鉴...
探讨肠道病毒71型(enterovirus 71，EV71)诱导人脑微血管内皮细胞(human brain microvascular endothelial cells，HBMECs)的凋亡及其相关机制。 方法 流式细胞仪检测EV71能否诱导HBMECs的凋亡；免疫荧光方法观察EV71诱导HBMECs线粒体膜电位的改变情况；免疫印迹检测DJ-1蛋白在EV71感染HBMEC...
评价痰涂片革兰染色在重症监护室（ICU）医院获得性肺炎（HAP）患者中早期临床诊断及指导用药的意义和价值。方法对某院ICU 2011年3月—2012年2月疑诊医院获得性肺炎的289例患者，经人工气道抽取下呼吸道痰标本，分别进行痰涂片革兰染色镜检和病原学培养，对培养出来的病原菌进行菌种鉴定，比较两种方法的检验结果。结果送检痰标本890份，其中合格标本790份，合格率为88.76%。共培养出病原菌52...
金黄色葡萄球菌转座文库构建与持留突变株研究
金黄色葡萄球菌 转座子文库 持留性
2014/5/21
利用Tn917转座子构建金黄色葡萄球菌（简称“金葡菌”）转座突变文库，从构建好的文库中筛选到持留性突变菌株。 方法 将含有Tn917的pID408质粒电转化入金葡菌Newman株，利用高温及红霉素诱导使其发生转座作用，建立转座子文库并对其进行质量评价；通过对稳定期24 h及36 h菌液予以100 MIC达托霉素处理，之后计数活菌数目的方法，比较文库中筛选到的特定菌株T18...